Green biosynthesis of phenylglyoxylic acid by biotransformation using recombinant Escherichia coli whole cells

被引：0

作者：

Tang C. ^{[1
,2
]}

Shi H. ^{[1
]}

He Z. ^{[1
]}

Ding P. ^{[1
]}

Jiao Z. ^{[1
]}

Kan Y. ^{[1
]}

Yao L. ^{[1
]}

机构：

[1] Henan Provincial Engineering Laboratory of Insect Bio-reactor and Henan Key Laboratory of Ecological Security for Water Source Region of Mid-line of South-to-North, Nanyang Normal University, Nanyang

[2] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai

来源：

Huagong Xuebao/CIESC Journal | 2018年 / 69卷 / 06期

关键词：

Biotransformation; Green chemistry; Mandelate dehydrogenase; Phenylglyoxylic acid; Whole cell catalysis;

D O I：

10.11949/j.issn.0438-1157.20171414

中图分类号：

学科分类号：

摘要：

Phenylglyoxylic acid (PGA) is key building block in the chemical synthesis, which could be used to synthesize a variety of important pharmaceutical intermediate, thus exploiting the green synthesis process of phenylacetone acid has significant economic value. The recombinant Escherichia coli whole cells containing the encoding gene of LhDMDH was used as catalyst in this study. The biotransformation of D-mandelic acid was researched under the condition of no coenzyme and cosubstrate addition. Subsequently, the transformed products were purified and identified. These results indicated that biosynthesis of phenylglyoxylic acid was realized under the condition of no coenzyme and cosubstrate addition. The yield and purity of PGA were 45% and 99%, respectively. In addition, this study established a solid foundation for chiral resolution of racemic mandelic acid and the biosynthesis of PGA. © 2018, Science Press. All right reserved.

引用

页码：2627 / 2631

页数：4

共 15 条

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