Label-free biosensing characteristics of micro/nano-fiber coupler

被引：0
|
作者：
Wang H. [1 ,2 ]
Zhou W. [1 ]
Li K. [1 ]
Hao P. [1 ]
Chi M. [1 ,2 ]
Wu Y. [1 ]
机构：
[1] State Key Laboratory of Applied Optics, Changchun Institute of Optics, Fine Mechanics and Physics, Chinese Academy of Sciences, Changchun, 130033, Jilin
[2] University of Chinese Academy of Sciences, Beijing
来源：
Guangxue Xuebao/Acta Optica Sinica | 2017年 / 37卷 / 03期
关键词：
Fiber optics; Fiber sensors; Label-free biodetection; Micro/nano-fiber coupler; Reusability;
D O I：
10.3788/AOS201737.0306005
中图分类号：
学科分类号：
摘要：
A portable biosensor with high sensitivity is proposed, and the biosensor is based on a micro/nano-fiber coupler. When the biosensor is used in label-free biodetection, its sensitivity and reusability are studied. A fiber coupler with the waist diameter of 3 μm is fabricated by the fused biconical taper method, and then the coupler is used in refractive index detection experiment. A refractive index sensitivity of 1402.3 nm/RIU is measured in experiment. The detection results are fitted, and the correlation coefficient of the fitted curve is 0.99459.The calculated results of the weak coupling model of the micro/nano-fiber coupler are in good agreement with the experimental results. The detection limit of 2 pg/mL is obtained when the micro/nano-fiber coupler is used to detect goat immune globulin (IgG) antigen. The reusability of the biosensor is verified by 10 dissociation experiments. These experiments demonstrate that the micro/nano-fiber coupler can obtain a high detection sensitivity in the label-free biosensing and has good practical value. © 2017, Chinese Lasers Press. All right reserved.
引用
收藏
页数：7
相关论文
共 21 条
  • [1] Huang H., Zhai J., Ren B., Et al., Fiber-optic evanescent wave biosensor and its application, Acta Optica Sinica, 24, 4, pp. 451-454, (2003)
  • [2] Wu X., Wang Y., Tong L., Optical microfibers and their applications, Physics, 44, 6, pp. 356-365, (2015)
  • [3] Liao C.R., Wang D.N., He X.Y., Et al., Twisted optical microfibers for refractive index sensing, IEEE Photonics Technolology Letters, 23, 13, pp. 848-850, (2011)
  • [4] Bo L., O'Mahony C.C., Semenova Y., Et al., Microfiber coupler based label-free immunosensor, Optics Express, 22, 7, pp. 8150-8155, (2013)
  • [5] Xia X., Cao Y., Development and application of biosensors, a review, Journal of Chongqing Vocational & Technical Institute, 17, 1, pp. 149-152, (2008)
  • [6] Natale C.D., Paolesse R., Macagnano A., Et al., Electronic nose and electronic tongue integration for improved classification of clinical and food samples, Sensors and Actuators B: Chemical, 64, 1-3, pp. 15-21, (2000)
  • [7] Leung A., Rijal K., Shankar P.M., Et al., Effects of geometry on transmission and sensing potential of tapered fiber sensors, Biosensors and Bioelectronics, 21, 12, pp. 2202-2209, (2006)
  • [8] Daneshvar M.A., Peralta J.M., Casay G.A., Et al., Detection of biomolecules in the near-infrared spectral region via a fiber-optic immunosensor, Journal of Immunological Methods, 226, 1, pp. 119-128, (1999)
  • [9] De Grandpre M.D., Burgess L.W., Long path fiber-optic sensor for evanescent field absorbance measurements, Analytical Chemistry, 60, 23, pp. 2582-2586, (1988)
  • [10] Leung A., Shankar P.M., Mutharasan R., Real-time monitoring of bovine serum albumin at femtogram/mL levels on antibody-immobilized tapered fibers, Sensors and Actuators B: Chemical, 123, 2, pp. 888-895, (2007)
123