Development of a mature fungal technology and production platform for industrial enzymes based on a Myceliophthora thermophila isolate, previously known as Chrysosporium lucknowense C1

被引：1

作者：

Visser H. ^{[1
]}

Joosten V. ^{[1
]}

Punt P.J. ^{[2
,3
]}

Gusakov A.V. ^{[4
]}

Olson P.T. ^{[5
]}

Joosten R. ^{[1
]}

Bartels J. ^{[1
]}

Visser J. ^{[6
]}

Sinitsyn A.P. ^{[7
]}

Emalfarb M.A. ^{[8
]}

Verdoes J.C. ^{[1
]}

Wery J. ^{[1
]}

机构：

[1] Dyadic Netherlands, 6709 PA Wageningen

[2] TNO Microbiology and Systems Biology, 3700 AJ Zeist

[3] University Leiden, Molecular Microbiology and Biotechnology, 2333 BE Leiden

[4] Department of Chemistry, M.V Lomonosov Moscow State University, Moscow 119991

[5] Bio-Technical Resources, Manitowoc, WI 54220

[6] Fungal Genetics and Technology Consultancy, 6700 AJ Wageningen

[7] A.N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow 119071, Leninsky Prospect

[8] Dyadic International, Inc., Jupiter, FL 33477-5094

来源：

Industrial Biotechnology | 2011年 / 7卷 / 03期

关键词：

biofuels; biorefineries; C; lucknowense; Chrysosporium lucknowense; filamentous fungi; industrial enzymes; M; thermophila; Myceliophthora thermophila; protein production; strain improvement;

D O I：

10.1089/ind.2011.7.214

中图分类号：

学科分类号：

摘要：

The filamentous fungus C1 was developed into an expression platform for screening and production of diverse industrial enzymes. C1 shows a lowviscosity morphology in submerged culture, enabling the use of complex growth and production media. This morphology furthermore allowed C1 to be used as a host for high-throughput robotic screening of gene libraries. A C1-genetic toolbox was developed, which enabled the generation of a large collection of dedicated C1 host strains and gene-expression strategies. The 38 Mbp genome was sequenced and found to be rich in biomass-hydrolyzing-enzyme-encoding genes. C1 production strains have been developed that produce large quantities of these enzyme mixtures (up to 100 g/L total protein). Recombinant C1 strains were constructed that produce single enzymes in a relatively pure form, facilitating enzyme purification and characterization, as well as for commercial applications. Molecular phylogenetic studies revealed that C1, previously classified as Chrysosporium lucknowense based on morphological characteristics, is actually a Myceliophthora thermophila isolate. In addition, C1 has proven to be a source of novel industrial enzymes, and the C1-technology platform developed has been applied as a tool for research on and production of industrial enzymes for various industrial applications, such as biofuels and biorefineries. © 2011 Mary Ann Liebert, Inc.

引用

页码：214 / 223

页数：9