Preparation of Kartogenin-Conjugated Chitosan-Hyaluronic Acid Hydrogel for Nucleus Pulposus Regeneration

被引：0
|
作者：
Luo Z.-Q. [1 ]
Wu Z.-S. [1 ]
Tan J. [1 ]
Jiang Y.-L. [1 ]
He X.-X. [1 ]
Zhu Y.-X. [1 ]
机构：
[1] Shenzhen Key Laboratory for Anti-ageing and Regenerative Medicine, Health Science Center, Shenzhen University, Shenzhen
来源：
Gao Xiao Hua Xue Gong Cheng Xue Bao/Journal of Chemical Engineering of Chinese Universities | 2018年 / 32卷 / 06期
关键词：
Chitosan; Hyaluronic acid; Hydrogel; Kartogenin; Nucleus pulposus;
D O I：
10.3969/j.issn.1003-9015.2018.06.025
中图分类号：
学科分类号：
摘要：
Injectable constructs for in vivo gelation have many advantages in the regeneration of degenerated nucleus pulposus. Injectable hydrogel consisting of chitosan (CS) and hyaluronic acid (HA) crosslinked by glycerol phosphate (GP) (with different CS:GP:HA ratios: 6:3:1,5:3:2,4:3:3,3:3:4, 2:3:5,1:3:6,V:V:V) was developed for kartogenin (KGN) delivery. In vitro gelation time, morphology, swelling ratio, weight loss, compressive modulus, cumulative release of KGN and biocompatibility were studied. Human adipose-derived stem cells (ADSCs) were encapsulated in the hydrogel to study the effects of KGN, TGF-β and KGN+TGF-β on stem cell proliferation and differentiation into nucleus pulposus-like cells. The results show that hydrogel with high HA concentration has shorter gelation time, higher water uptake, faster mass loss and KGN release. KGN-conjugated hydrogel with CS:GP:HA ratio of 5:3:2 show optimal cell proliferation and differentiation. PCR results show significant difference between hydrogel with and without KGN/TGF-β. However, there is no significant difference for nucleus pulposus induced by KGN and/or TGF-β. In conclusion, a KGN conjugated CS-HA hydrogel (5:3:2) for sustained release of KGN is developed for promoting ADSCs proliferation and nucleus pulposus differentiation, which is useful for the repair of degenerative NP tissues after minimally invasive surgery. © 2018, Editorial Board of "Journal of Chemical Engineering of Chinese Universities". All right reserved.
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页码：1427 / 1434
页数：7
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