The two-step synthesis of 1,3-oleoyl-2-palmitoylglycerol by Candida sp. 99–125 lipase

被引：0

作者：

Liu C. ^{[1
]}

Zhang Y. ^{[1
]}

Zhang X. ^{[1
]}

Nie K. ^{[1
,2
]}

Deng L. ^{[1
,2
]}

Wang F. ^{[1
]}

机构：

[1] Beijing Bioprocess Key Laboratory, State Key Laboratory of Chemical Resource Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing

[2] Amoy—BUCT Industrial Bio-technovation Institute, Amoy

来源：

Nie, Kaili (niekl@mail.buct.edu.cn) | 2016年 / Elsevier B.V., Netherlands卷 / 133期

关键词：

1,3-oleoyl-2-palmitoylglycerol; Alcoholysis; Candida sp. 99–125; Esterification; Monopalmitin;

D O I：

10.1016/j.molcatb.2016.10.005

中图分类号：

学科分类号：

摘要：

This paper proposed a two-step method to synthesize 1,3-Oleyl-2-Palmitoylglycerol (OPO) which is an important ingredient in the infant formula using an economical lipase from Candida sp. 99–125. In the first step, tripalmitin (PPP) is enzymatically alcoholyzed to monopalmitin (2-MP) by Candida sp. 99–125 lipase. MP is then recovered through solvent extraction at 78.03% yield and 98.01% purity, which are better than crystallization purification (75.85% yield and 86.37% purity). In the second step, the recovered 2-MP is solvent-free esterified with oleic acid, using the same lipase. OPO was obtained at a yield of 65%, including 75% palmitic acid in the Sn-2 position. This study has shown that the two-step method could give a higher yield of OPO than the previously reported one-step method using economical lipase. © 2016

引用

页码：S1 / S5

页数：4

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