Preparation of a novel thiolated human-like collagen iron

In order to improve Human-like Collagen (HLC) iron binding capacity, the thiolated Human-Like collagen (SH-HLC) was adopted as chelating substrate. The binding of iron to SH-HLC was examined at varying pH, molar ratio (SH-HLC/Fe) and reaction time. The maximum binding capacity of SH-HLC was 31.18 mol iron per mol SH-HLC at pH 7.5, molar ratio 1:500 and reaction time 40 min. In addition, the SH-HLC and human-like collagen iron (SH-HLC-Fe) complex showed no toxicity to BHK cell through cell viability assay. These results suggest that the SH-HLC-Fe complex is a potential compound for use as a iron source. © 2013 Asian Network for Scientific Information.