Studies on the Combined Effects of Aflatoxin B1 and Microcystin LR on Liver Injury/Liver Cancer in Male Wild-type C57BL/6 Mice at Low Dose and Sub-chronic Condition

被引：0
|
作者：
Li Y. [1 ,2 ,3 ]
Sun X. [1 ,3 ]
Wang J. [1 ,3 ]
机构：
[1] School of Food Science and Technology, Jiangnan University, Wuxi
[2] Institute of Quality Standards and Testing Technology for Agro-products Quality and Safety, Chinese Academy of Agricultural Sciences, Beijing
[3] State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi
来源：
Journal of Chinese Institute of Food Science and Technology | 2021年 / 21卷 / 08期
关键词：
Aflatoxin B[!sub]1[!/sub; Combined effects; Liver cancer; Liver injury; Microcystin LR;
D O I：
10.16429/j.1009-7848.2021.08.013
中图分类号：
学科分类号：
摘要：
Objective: This paper aimed to evaluate the probability of liver cancer combination and intensity of combined liver injury induced by AFB1+MC-LR at low dose and sub-chronic conditions, and to promote the understanding the threshold and occurrence possibility of liver toxicity co-exposure to AFB1+MC-LR at dietary scenarios, the results were helpful for risk control(e.g. MRLs setting). Methods: Based on the male wild-type C57BL/6 mice, CI model combined with scenarios extrapolating was used to predict the combined risk of hepatoma induced by AFB1+MC-LR at low dose and sub-chronic condition. And then, according to the expression of 5 typical targets in serum and liver, the combined effects of liver injury were explored. Results: Within dose range of 0-20 mg/kg bw, the CI index was in the range of 0-1.5, which showed the probability of additive/synergistic effect was relatively higher. With the equal effect ratio, the overall expression of combined group related with five indicators was slightly higher than that of single component in the serum and liver (P<0.05), which was also confirmed by principal component analysis, the probability of synergistic effect was relatively higher than antagonistic and simple additive effects. With unequal effect ratio, except TBIL and AFP in serum, the change of any single component will affect the expression of combined toxicity (P<0.05), while the change of single dose in liver will significantly affect the expression of combined toxicity (P<0.001). Conclusion: The results showed that early liver injury induced by AFB1+MC-LR was slight synergistic effect at low dose and sub-chronic condition, and the change of any single mycotoxin can affect the expression of combined liver injury in mixtures. It should be paid attention to in the standard making and supervision. © 2021, Editorial Office of Journal of CIFST. All right reserved.
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页码：136 / 151
页数：15
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