Optimization of Chitinase Production by the Photobacterium sp. LG-1

被引：0

作者：

Chen L.-G. ^{[1
,2
]}

Wu J.-W. ^{[1
,2
]}

Zhang R. ^{[1
,2
]}

Zhang Q.F. ^{[1
,2
]}

Chi N.Y. ^{[1
,2
]}

Wang X.H. ^{[1
,2
]}

机构：

[1] School of Life Science and Biotechnology, Dalian University, Dalian

[2] Liaoning Marine Microbial Engineering Technology Research Center, Dalian

来源：

Science and Technology of Food Industry | 2021年 / 42卷 / 01期

关键词：

chitinase; optimization of fermentation conditions; Photobacterium; response surface methodology;

D O I：

10.13386/j.issn1002-0306.2019120161

中图分类号：

学科分类号：

摘要：

To increase chitinase activity, response surface metliodology was used to optimize the fermentation conditions of cold -adapted chitinase by Photobacterium sp.LG-1.The result of Placket-Burman showed that the important factors influencing chitinase production were fermentation temperature, fermentation time and yeast extract. The optimal fermentation conditions were determined as followed: colloidal chitin 12.0 g/L, yeast extract 4.5 g/L, shaking speed 220 r/min, fermentation temperature 20 °C, liquid volume 75 mL/250 mL,inoculums dose 1% " pH7.0"and fermentation time 120 h,respectively. Under these conditions, the max enzyme activity of chitinase was 5.10 U/mL.The maximum theoretic value was consistent with mean value of verification test and the chitinase production was increased by 11.50% comparing to that before optimization.lt provides a reference for the research on the degradation mechanism of low - temperature chitinase and its application in industry. © The Author(s) 2021.

引用

页码：110 / 114and131

共 28 条

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