Design of variable light-sheet illumination system

被引：0
|
作者：
Jiang L. [1 ,2 ]
Rui C. [1 ,2 ]
Zeng C. [1 ,2 ]
Ma S. [1 ,2 ]
机构：
[1] School of Optoelectronic Science and Engineering, Soochow University, Suzhou
[2] Key Lab of Advanced Optical Manufacturing Technologies of Jiangsu Province, Key Lab of Modern Optical Technologies of Education Ministry of China, Suzhou
来源：
Hongwai yu Jiguang Gongcheng/Infrared and Laser Engineering | 2019年 / 48卷 / 11期
关键词：
Afocal zoom expanding; Illumination uniformity; Optical design; Variable light-sheet illumination system;
D O I：
10.3788/IRLA201948.1114003
中图分类号：
学科分类号：
摘要：
To solve the problem of the single thickness of the light-sheet fluorescence microscopy, the design of the variable light-sheet illumination system was carried out based on zoom beam expanding. First, each group of the light-sheet illumination system was calculated using Gaussian optics to obtain the relation between the thickness of the light-sheet and the expansion ratio as well as the relation among the expansion ratio, the lateral magnification and focal length of each group. Then a variable light-sheet illumination system based on 10 ×beam expanding was designed to generate the light-sheet with continuously variable thickness and length. Finally, the parameters and uniformity of the light-sheet and the system tolerance were analyzed. The results show that the thickness of the continuously variable light-sheet is 3.33 μm to 33.3 μm, and the illumination uniformity is 0.65, 0.4 and 0.61 on the YOZ plane within 60% light-sheet height at low (1 ×), medium (6 ×) and high (10 ×) expansion ratios, respectively. Tolerance analysis shows that the maximum change of the light-sheet thickness is less than the 15% of design value at expansion ratio of 1×, less than 6% at 6×and 10×. The design realizes the continuous variation of the light sheet thickness, and the region of 60% light-sheet height is favorable for the observation of the sample. © 2019, Editorial Board of Journal of Infrared and Laser Engineering. All right reserved.
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