Determination of 30 Kinds of Antiparasitic Drugs in Animal-derived Foods by QuEChERS-UPLC-MS/MS

被引：0

作者：

Feng L. ^{[1
]}

Huang Q. ^{[1
]}

Ye M. ^{[1
]}

Huang Y. ^{[1
]}

Lin H. ^{[1
]}

Liang M. ^{[1
]}

Xu H. ^{[1
]}

机构：

[1] Fujian Inspection and Research Institute for Product Quality, China National Quality Supervision and Testing Center for Processed Food, Fuzhou

来源：

Science and Technology of Food Industry | 2024年 / 45卷 / 06期

关键词：

animal derived food; antiparasitic drugs; drug residues; QuEChERS; ultra-high-performance liquid chromatography-tandem mass spectrometry;

D O I：

10.13386/j.issn1002-0306.2023050175

中图分类号：

学科分类号：

摘要：

Objective: A method for determining the residues of 30 antiparasitic drugs in animal-derived food using ultrahigh-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was established. Methods: The samples were extracted with acetonitrile and 1% ammonia acetic ether and purified by QuEChERS. After purification, Waters ACQUITY UPLCTM BEH C18 column was used for separation, and a gradient elution of 10 mmol/L ammonium formate (containing 0.1% formic acid) aqueous solution, acetonitrile:methanol (50:50, v:v) was performed as the mobile phase. Detection was carried out using electrospray ionization (ESI) in both positive and negative ion modes using multiple reaction monitoring (MRM). Matrix-matched external standard quantification was used. Results: Under the optimized conditions, the 30 antiparasitic drugs showed good linearity within their respective linear ranges, with coefficient of determination (r2) greater than 0.99. The recoveries ranged from 70.1% to 111%, and the relative standard deviations were between 0.10% and 9.1% (n=6). The method detection limit ranged from 0.001 to 0.3 μg/kg, and the method quantification limit ranged from 0.004 to 1 μg/kg. Conclusion: The method is sensitive, accurate, and exhibits good repeatability and stability, making it suitable for detecting various antiparasitic drug residues in animal-derived food. © 2024 Editorial Department of Science and Technology of Food Science. All rights reserved.

引用

页码：280 / 288

页数：8

共 30 条

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