Multiplex Ligation-Dependent Probe Amplification for Simultaneous Detection of Six Allergenic Ingredients in Foods

被引：0

作者：

Wang M. ^{[1
]}

Liu Y. ^{[1
]}

Li S. ^{[1
]}

Dong W. ^{[2
]}

Zhang T. ^{[1
]}

Lin J. ^{[1
]}

Zhu B. ^{[1
]}

Zhang L. ^{[1
]}

机构：

[1] Key Laboratory of Detection Technology of Focus Chemical Hazards in Animal-derived Food for State Market Regulation, Hubei Provincial Engineering and Technology Research Center for Food Quality and Safety Test, Hubei Provincial Institute for Food Supervisi

[2] Wuhan Vocational College of Software and Engineering, Wuhan

来源：

Shipin Kexue/Food Science | 2023年 / 44卷 / 04期

关键词：

food allergen; limit of detection; multiple detection; multiplex ligation-dependent probe amplification;

D O I：

10.7506/spkx1002-6630-20220127-283

中图分类号：

学科分类号：

摘要：

A multiplex ligation-dependent probe amplification (MLPA) technique was developed for simultaneous detection of six food allergens: soy, sesame, peanut, almond, hazelnut and walnut. Six pairs of species-specific MLPA probes targeting the internal transcribed spacer (ITS) sequences were designed and synthesized. The probes were hybridized with the target DNA, ligated and amplified by polymerase chain reaction (PCR) and the amplicons were detected using capillary electrophoresis, revealing that they could clearly discriminate the six food allergens. Good specificity of the MLPA system was observed for more than 20 plants, animals and bacteria. The limit of detection (LOD) of the assay was determined to be 5 mg/kg for model cookie spiked with different concentrations of the target species. The applicability of the MLPA assay was demonstrated through the analysis of 30 different commercial products, evidencing good performance in practical applications. Therefore, the MLPA technique is highly specific and sensitive and can provide technical support for the assessment, label management and risk control of food allergens. © 2023 Chinese Chamber of Commerce. All rights reserved.

引用

页码：300 / 305

页数：5

共 25 条

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