Effect of Quorum Sensing Systems on Biofilm Formation by Listeria monocytogenes

被引：1

作者：

Zhang X. ^{[1
]}

Lu Z. ^{[1
]}

Zheng L. ^{[1
]}

Lü Z. ^{[1
]}

Zhou L. ^{[1
]}

Meng F. ^{[1
]}

Bie X. ^{[1
]}

机构：

[1] College of Food Science and Technology, Nanjing Agricultural University, Nanjing

来源：

Shipin Kexue/Food Science | 2022年 / 43卷 / 18期

关键词：

AgrD gene; Biofilm; Homologous recombination; Listeria monocytogenes; LuxS gene;

D O I：

10.7506/spkx1002-6630-20211002-006

中图分类号：

学科分类号：

摘要：

This work was undertaken to study the regulatory effects of the Agr and LuxS/AI-2 quorum sensing systems on biofilm formation by Listeria monocytogenes (Lm). The agrD and luxS genes of LMB33426 were knocked out by homologous recombination, and the biofilm formation characteristics of the wild-type and mutant strains were investigated comparatively. The results showed that the biofilm formation ability of ΔagrD and ΔluxS was reduced compared with that of the wild-type strain. The hydrophobicity of ΔagrD decreased significantly, and its swimming mobility was higher than that of the wild-type strain at 37 ℃. However, no significant difference was found in drug resistance between the wild-type and mutant strains. This study provides a basis for further research on the regulatory mechanism of quorum sensing systems on biofilm formation by Lm and more broadly, for the development of novel strategies for the prevention and control of Lm infections. © 2022, China Food Publishing Company. All right reserved.

引用

页码：105 / 112

页数：7

共 25 条

[1] LIU Y T, SUN W X, SUN T M, Et al., The prevalence of Listeria monocytogenes in meat products in China: a systematic literature review and novel meta-analysis approach, International Journal of Food Microbiology, 312, (2020)
[2] HYDEN P, PIETZKA A, LENNKH A, Et al., Whole genome sequence-based serogrouping of Listeria monocytogenes isolates, Journal of Biotechnology, 235, SI, pp. 181-186, (2016)
[3] DA SILVA E P, DE MARTINIS E C P., Current knowledge and perspectives on biofilm formation: the case of Listeria monocytogenes, Applied Microbiology and Biotechnology, 97, 3, pp. 957-968, (2013)
[4] ABDALLAH M, BENOLIEL C, DRIDER D, Et al., Biofilm formation and persistence on abiotic surfaces in the context of food and medical environments, Archives of Microbiology, 196, 7, pp. 453-472, (2014)
[5] OLOKETUYI S F, KHAN F., Inhibition strategies of Listeria monocytogenes biofilmscurrent knowledge and future outlooks, Journal of Basic Microbiology, 57, 9, pp. 728-743, (2017)
[6] LUO Q, SHANG J L, FENG X Q, Et al., PrfA led to reduced biofilm formation and contributed to altered gene expression patterns in biofilm-forming Listeria monocytogenes, Current Microbiology, 67, 3, pp. 372-378, (2013)
[7] LEE Y J, WANG C L., Links between S-adenosylmethionine and Agr-based quorum sensing for biofilm development in Listeria monocytogenes EGD-e, Microbiologyopen, 9, (2020)
[8] AUTRET N, RAYNAUD C, DUBAIL I, Et al., Identification of the agr locus of Listeria monocytogenes: role in bacterial virulence, Infection and Immunity, 71, 8, pp. 4463-4471, (2003)
[9] PASPALIARI D K, MOLLERUP M S, KALLIPOLITIS B H, Et al., Chitinase expression in Listeria monocytogenes is positively regulated by the Agr system, PLoS ONE, 9, (2014)
[10] ZETZMANN M, BUCUR O I, CRAUWELS P, Et al., Characterization of the biofilm phenotype of a Listeria monocytogenes mutant deficient in Agr peptide sensing, Microbiologyopen, 8, (2019)

← 1 2 3 →