Kinetic modeling and process optimization of avilamycin production

Batch fermentation of avilamycin was studied in a 30 L fermentor using Streptomyces viridochromogenes as the fermentation strain, and single-factor methodology was applied to optimize culture conditions. Kinetics of the optimized fermentation process was analyzed. The results show that maximum avilamycin productivity of 1 571.04 mg∙L-1 can be achieved at 228 h when the rotational speed = 320 r∙min-1, voluntary ventilation = 2.0 vvm and initial pH = 7.5 with 0.1% L-Val added every 24 hours. Three mathematical models were established to describe the kinetics of the avilamycin production,which were based on Logistic equation for cell growth, Luedeking-Piret equation for avilamycin production and mass balance equation for total sugar consumption. The fitting degrees between the calculated values and the experimental data were 0.989 6, 0.990 9 and 0.981 9 respectively, which indicates that the models can reflect the avilamycin batch fermentation processes and provide theoretical basis for further optimization of fermentation process and on-line monitoring. © 2019, Editorial Board of "Journal of Chemical Engineering of Chinese Universities". All right reserved.