Purpose This paper presents a transparent and rigorous methodological approach to conducting a case study of social performance (SP) within the conventional life cycle of Canadian beef. The objective was to explore the potential benefits and risks (or hotspots) of practices on Canadian beef farm businesses (BFBs) and slaughter operations (BSOs) to establish valid benchmarks and a social sustainability roadmap for decision-makers to improve SP. Methods This study undertook a novel and participatory approach to social life cycle assessment (S-LCA). Respondents from both within and at-arms-length to the beef value chain were engaged to identify SP practices and perceptions and develop the life cycle inventory. The goal and scope, inventory, assessment, and interpretation were conducted in a three-phased approach: (i) scoping; (ii) practice-based assessment; and (iii) deep-dive assessment. Data collected through mixed methods, including Q method, interviews, surveys, and literature review, were assessed using a type I (reference scale) approach and interpreted through critical interpretive synthesis. Organizational SP was explored at BFBs and BSOs, considering the following stakeholders: value chain actors, farmers, employees, and cattle. Results and discussion Outcomes from the applied approach explore the interrelations between organizations and stakeholders along the value chain. Impact categories concerning labour management, people's health and safety, and animal care were prioritized for deep-dive assessment. Subcategories included novel topics, like recruitment and retention and access to mental health resources. A range of potential benefits and areas for improving SP were identified at BFBs and BSOs. Potential risks from SP were identified for stakeholders from working hours, communication and dispute resolution, animal transport, and personal protective equipment practice areas. Results informed strategic activities for the National Beef Sustainability Strategy. Conclusion This study contributes to the social sustainability discourse in livestock systems by demonstrating a qualitative approach to S-LCA that can be replicated by practitioners to explore valid and locally specific social dimensions of sustainability. Practitioners may consider the approach and results in future studies to better capture and manage the complex and dynamic nature of livestock systems, leading to more effective social sustainability decisions that incorporate diverse stakeholder perspectives.