Cubic liquid crystals containing propolis flavonoids as in situ thermo-sensitive hydrogel depots for periodontitis treatment: Preparation, pharmacodynamics and therapeutic mechanisms

被引：2

作者：

Tang, Maomao ^{[1
]}

Li, Jiaxin ^{[1
]}

Wang, Guichun ^{[1
]}

Wang, Yuxiao ^{[1
]}

Peng, Chengjun ^{[1
]}

Chang, Xiangwei ^{[1
,2
,3
,4
]}

Tao, Yaotian ^{[1
]}

Guo, Jian ^{[1
,2
,3
,4
]}

Gui, Shuangying ^{[1
,2
,3
,4
,5
,6
]}

机构：

[1] Anhui Univ Chinese Med, Coll Pharm, Hefei, Anhui, Peoples R China

[2] Anhui Acad Chinese Med, Inst Pharmaceut, Hefei, Anhui, Peoples R China

[3] Anhui Prov Key Lab Pharmaceut Preparat Technol & A, Hefei, Anhui, Peoples R China

[4] Anhui Educ Dept AUCM, Engn Technol Res Ctr Modernized Pharmaceut, Hefei, Anhui, Peoples R China

[5] MOE, Anhui Joint Collaborat Innovat Ctr Qual Improvemen, Hefei, Anhui, Peoples R China

[6] Anhui Univ Chinese Med, Ctr Xinan Med & Modernizat Tradit Chinese Med IHM, Hefei, Anhui, Peoples R China

来源：

EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES | 2024年 / 196卷

基金：

中国国家自然科学基金;

关键词：

Cubic liquid crystal; Periodontitis; Hydrogel; Propolis flavonoid; Tissue regeneration; DELIVERY; ARTHRITIS; GELS;

D O I：

10.1016/j.ejps.2024.106762

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Propolis has a long ethnopharmacological history for oral periodontal diseases treatment. Propolis flavonoids are main active components for anti-inflammation and tissue protection. However, the intractable dissolution properties of propolis flavonoids and complex oral environment pose great challenges for periodontal delivery. In addition, the therapeutic mechanism as well as the therapeutic correlation of inflammation resolution and tissue regeneration remain unclear for propolis flavonoids. In this study, we constructed an in situ thermosensitive depot systems using total flavonoids from propolis-loaded cubic liquid crystals (TFP-CLC) hydrogel for periodontal delivery. TFP-CLC inhibited inflammatory cell infiltration, reactive oxygen species and the expression of inflammatory cytokines of NF-kappa B and IL-1 beta. In addition, alveolar bone and collagen were significantly regenerated after TFP-CLC administration according to micro-CT and immunohistochemistry. Mechanism studies suggested that TFP-CLC alleviated inflammation and promoted alveolar bone repair via regulating TLR4/ MyD88/NF-kappa B p65 and RANK/NF-kappa B signaling pathways, respectively. Correlation analysis further confirmed that the inflammatory resolution produced by TFP-CLC could accelerate periodontal tissue regeneration. In summary, TFP-CLC is a promising multifunctional in situ thermo-sensitive hydrogel depots for periodontitis treatment.

引用

页数：11