Artemisia vulgaris Extract as a Novel Therapeutic Approach for Reversing Diabetic Cardiomyopathy in a Rat Model

Diabetic cardiomyopathy, a severe diabetic complication, impairs heart function, leading to heart failure. Treatment that effectively addresses this condition without causing side effects is urgently needed. Current anti-hyperglycemic therapies are expensive, has side effects and do not effectively prevent cardiac remodeling. Therefore, it is important to explore natural products that may have the potential to reverse cardiac remodeling. That is why the aim of the current study was to determine the left ventricular remodeling potential of the methanolic extract of Artemisia vulgaris in a diabetic cardiomyopathy rat model. Following the initial comprehensive phytochemical evaluation of plant phenolic and flavonoid content, which showed strong anti-hyperglycemic and antioxidant activities, an extract of Artemisia vulgaris was administered in an in vivo experiment. Diabetic cardiomyopathy was induced in Wistar albino rats according to previously described protocols in the literature, and the effect of treatment was checked by serum and histopathological analysis after 45 days. Artemisia vulgaris treatment significantly (p <= 0.05) reduced fasting blood glucose (108.5 +/- 1.75 mg/dL), glycated hemoglobin (4.03 +/- 0.12 %), serum glucose (116.66 +/- 3.28 mg/dL), insulin (15.66 +/- 0.66 ng/mL), total oxidant status (54.66 +/- 3.22 mu mol H(2)O(2)Equiv.L-1), Malondialdehyde (0.20 +/- 0.01 mmol/L), total cholesterol (91.16 +/- 3.35 mg/dL), triglycerides (130.66 +/- 3.15 mg/dL), low-density lipids (36.57 +/- 1.02 mg/dL), sodium (140 +/- 3.21 mmol/L), calcium (10.44 +/- 0.24 mmol/L), creatine kinase MB (1227.5 +/- 17.89 IU/L), lactate dehydrogenase (1300 +/- 34.64 IU/L), C-reactive protein (30 +/- 0.57 pg/mL), tumor necrosis factor-alpha (58.66 +/- 1.76 pg/mL), atrial natriuretic peptide (2.53 +/- 0.04 pg/mL), B-type natriuretic peptide (10.66 +/- 0.44 pg/mL), aspartate aminotransferase (86.5 +/- 4.99 U/L), Alanine Transaminase (55.33 +/- 2.90 U/L), urea (25.33 +/- 1.15 mg/dL) and creatinine (0.64 +/- 0.02 mg/dL) but significantly increased (p <= 0.05) total antioxidant capacity (1.73 +/- 0.07 mmol Trolox Equil./L), high-density lipids (40 +/- 1.59 mg/dL) and potassium (3.82 +/- 0.04 mmol/L) levels. ECG and histopathology confirmed the significant improvement in remodeling and the reversal of structural changes in the heart and pancreas. In conclusion, Artemisia vulgaris possesses significant left ventricular remodeling potential in course of diabetes-induced cardiomyopathy.