Synthesis, characterization, and biomedical potential of nickel oxide and Silver-doped nickel oxide nanoparticles via green synthesis method

The present study focuses on the synthesis of pure and different concentrations, viz. 2%, 5%, and 10%, 15% of Silver doped nickel oxide (NiO) nanoparticles prepared by green synthesis method using aqueous flower extract of Moringa oleifera Lam. (MO). The flower extract of MO contains several bioactive constituents that can be used as a strong reducing, stabilizing and capping agent to prepare pure and Silver (Ag) doped NiO NPs. The XRD studies confirm the crystallinity structure of the material. Pure NiO is found to be around 20.38 nm on average, 14.7-18.29 nm for Ag-doped NiO nanoparticles, and it belongs to the face-centred cubic (FCC) structure. The morphology of the samples is studied using SEM analysis, and the elements present are confirmed by EDS. FTIRATR studies confirm the structure of the functional groups present in the samples. The UV-Vis spectra analyses are used to calculate the band gap values for the synthesized nanoparticles. Agar well diffusion methods are used to study the antimicrobial activity of green synthesized pure and silver-doped NiO nanoparticles. The antimicrobial studies reveal that good antibacterial activity is present in both gram-positive and gram-negative organisms of the green synthesis of pure and Silver doped NiO nanoparticles. Hence, the synthesized silver-doped NiO nanoparticles can be used for biomedical applications due to their antibacterial properties.