RhoGDI1 regulates cell-cell junctions in polarized epithelial cells

被引:1
|
作者
Wibbe, Nicolina [1 ]
Steinbacher, Tim [1 ]
Tellkamp, Frederik [2 ,3 ]
Beckmann, Niklas [1 ]
Brinkmann, Frauke [4 ]
Stecher, Manuel [4 ]
Gerke, Volker [4 ,5 ]
Niessen, Carien M. [2 ,6 ]
Ebnet, Klaus [1 ,5 ]
机构
[1] Univ Munster, Inst Associated Res Grp Cell Adhes & Cell Polar, Inst Med Biochem, Zent Molekularbiol Entzundung, Munster, Germany
[2] Univ Cologne, Univ Hosp Cologne, Dept Cell Biol Skin, Cologne, Germany
[3] Univ Cologne, Cologne Excellence Cluster Cellular Stress Respons, Cologne, Germany
[4] Univ Munster, ZMBE, Inst Med Biochem, Munster, Germany
[5] Univ Munster, Cells Inmot Cluster Excellence EXC 1003-CiM, Munster, Germany
[6] Univ Cologne, Univ Hosp Cologne, Ctr Mol Med Cologne CMMC, Cologne, Germany
关键词
ARHGDIA; epithelial barrier; contact inhibition of locomotion; JAM-A; RhoGDI1; tight junction; ADHESION MOLECULE JAM; PROTEIN-KINASE-C; CONTACT INHIBITION; E-CADHERIN; COLLECTIVE MIGRATION; SMALL GTPASES; VE-CADHERIN; RHO; COMPLEX; RAC1;
D O I
10.3389/fcell.2024.1279723
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Cell-cell contact formation of polarized epithelial cells is a multi-step process that involves the co-ordinated activities of Rho family small GTPases. Consistent with the central role of Rho GTPases, a number of Rho guanine nucleotide exchange factors (GEFs) and Rho GTPase-activating proteins (GAPs) have been identified at cell-cell junctions at various stages of junction maturation. As opposed to RhoGEFs and RhoGAPs, the role of Rho GDP dissociation inhibitors (GDIs) during cell-cell contact formation is poorly understood. Here, we have analyzed the role of RhoGDI1/ARHGDIA, a member of the RhoGDI family, during cell-cell contact formation of polarized epithelial cells. Depletion of RhoGDI1 delays the development of linear cell-cell junctions and the formation of barrier-forming tight junctions. In addition, RhoGDI1 depletion impairs the ability of cells to stop migration in response to cell collision and increases the migration velocity of collectively migrating cells. We also find that the cell adhesion receptor JAM-A promotes the recruitment of RhoGDI1 to cell-cell contacts. Our findings implicate RhoGDI1 in various processes involving the dynamic reorganization of cell-cell junctions.
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页数:17
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