1,25-(OH) 2 D 3 promotes hair growth by inhibiting NLRP3/IL-1,B and HIF-1 a /IL-1,B signaling pathways

Vitamin D is a crucial vitamin that participates in various biological processes through the Vitamin D Receptor (VDR). While there are studies suggesting that VDR might regulate hair growth through ligand-independent mechanisms, the efficacy of Vitamin D in treating hair loss disorders has also been reported. Here, through in vivo experiments in mice, in vitro organ culture of hair follicles, and cellular-level investigations, we demonstrate that 1,25(OH) 2 D 3 promotes mouse hair regeneration, prolongs the hair follicle anagen, and enhances the proliferation and migration capabilities of dermal papilla cells and outer root sheath keratinocytes in a VDR-dependent manner. Transcriptome analysis of VDR-knockout mouse skin reveals the involvement of HIF1 a , NLRP3, and IL-1 ,B in these processes. Finally, we confirm that 1,25-(OH) 2 D 3 can counteract the inhibitory effects of DHT on hair growth. These findings suggest that 1,25-(OH) 2 D 3 has a positive impact on hair growth and may serve as a potential therapeutic agent for androgenetic alopecia (AGA). (c) 2024 Elsevier Inc. All rights are reserved, including those for text and data mining, AI training, and similar technologies.