Phenotypic characterization and molecular identification of Bacillus pumilus isolated from Algerian goat's milk and evaluation of its synbiotic effect with Hammada scoparia L. extract and ZnO-NPs

被引：0

作者：

Chaima, Benine ^{[1
,2
]}

Boutlelis, Djahra Ali ^{[1
,2
]}

Touhami, Laiche Ammar ^{[3
,4
]}

Ghemam Amara, Djilani ^{[2
]}

Khemakhem, Maha Mezghani ^{[5
]}

Naccache, Chahnez ^{[5
]}

Atanassova, Maria ^{[6
]}

Zahnit, Wafa ^{[7
]}

Ahmad, Sheikh F. ^{[8
]}

Messaoudi, Mohammed ^{[9
]}

机构：

[1] Univ El Oued, Fac Life & Nat Sci, Dept Cellular & Mol Biol, El Oued 39000, Algeria

[2] Univ El Oued, Lab Biol Environm & Hlth LBEH, El Oued, Algeria

[3] Univ El Oued, Fac Life & Nat Sci, Dept Biol, ElOued, Algeria

[4] Univ El Oued, Lab Biodivers & Applicat Biotechnol Agr BABDA, El Oued, Algeria

[5] Univ Tunis El Manar, Fac Sci Tunis, Dept Biol, Lab Biochem & Biotechnol LR01ES05, Tunis, Tunisia

[6] Univ Chem Technol & Met, Sci Consulting, Chem Engn, Sofia, Bulgaria

[7] Univ Ouargla, Fac Math & Matter Sci, Lab Valorizat & Promot Saharan Resource VPRS, Ouargla, Algeria

[8] King Saud Univ, Coll Pharm, Dept Pharmacol & Toxicol, Riyadh, Saudi Arabia

[9] Nucl Res Ctr Birine, Ain Oussera 17200, Algeria

来源：

INTERNATIONAL JOURNAL OF FOOD PROPERTIES | 2024年 / 27卷 / 01期

关键词：

Goat's milk; probiotics; 16S rRNA; H; scoparia; ZnO-NPs; prebiotic activity; IN-VITRO; PHOTOCATALYTIC DEGRADATION; GREEN SYNTHESIS; ANTIOXIDANT; ANTIBACTERIAL; CALCINATION; MICROBIOTA; BACTERIA; SUBTILIS; GROWTH;

D O I：

10.1080/10942912.2024.2391399

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

This study was conducted to identify and characterize the Bacillus strain from Goat's milk in Algeria and to evaluate the prebiotic effect of H. scoparia aqueous extract and ZnO-NPs on the isolated Bacillus strain. Potentially probiotic bacteria were isolated and identified based on phenotypic characteristics, including the morphological, physiological (growth temperature, salt tolerance), biochemical (API 10S strip kits), and confirmed by 16S rDNA gene sequencing. Bacillus pumilus specie of lactic acid bacteria were identified. The base pair length of amplified DNA for the isolate was 1500 Bp. The isolates could not grow at 10 degrees C and 45 degrees C and tolerate up to 6% salt. H. scoparia extract and ZnO-NPs have a beneficial effect on the Bacillus pumilus strain for acid tolerance, bile tolerance, and cell surface hydrophobicity respectively; H. scoparia extract and ZnO-NPs results are high comparing to the control group's results. As the concentration of the tested samples increases, the percentage of probiotic characteristics of Bacillus pumilus increases, in contrast to the control sample. Therefore, they could be useful tools to define adequate combinations in colonic foods development and health benefits. The beneficial physiological and biochemical properties of isolated Bacillus pumilus space from Goat's milk with the H. scoparia aqueous extract and ZnO-NPs revealed their potential applications in the food industry.

引用

页码：1215 / 1229

页数：15