Protocol for isolating extracellular vesicles from pancreatic cancer cells for liver metastatic niche research

被引：0

作者：

Casabianca, Anthony ^{[1
]}

Bellina, Melanie ^{[3
,4
]}

Dudgeon, Crissy ^{[5
]}

Nezhad, Muhammad Sadeqi ^{[7
]}

Harris, Chris ^{[1
,2
]}

Fiore, Stephany ^{[4
]}

Bernet, Agnes ^{[3
,4
]}

Narrow, Wade ^{[1
,2
]}

Prela, Orjola ^{[1
]}

Wang, Lan ^{[6
]}

Astsaturov, Igor ^{[8
]}

Mehlen, Patrick ^{[3
,4
]}

Carpizo, Darren R. ^{[1
,2
]}

机构：

[1] Univ Rochester, Sch Med & Dent, Dept Surg, Div Surg Oncol, Rochester, NY 14620 USA

[2] Univ Rochester, Wilmot Canc Ctr, Rochester, NY 14620 USA

[3] Univ Lyon, Univ Claude Bernard Lyon1, Inst Convergence PLAsCAN, Ctr Rech Cancerol Lyon CRCL,INSERM U1052,CNRS UMR5, F-69008 Lyon, France

[4] Netris Pharma, F-69008 Lyon, France

[5] Rutgers Canc Inst New Jersey, New Brunswick, NJ 08901 USA

[6] Univ Rochester, Sch Med & Dent, Dept Pathol & Lab Med, Rochester, NY 14620 USA

[7] Univ Rochester, Clin & Translat Sci Inst, Rochester, NY USA

[8] Fox Chase Canc Ctr, Dept Med Oncol, Philadelphia, PA 19111 USA

来源：

STAR PROTOCOLS | 2024年 / 5卷 / 03期

关键词：

D O I：

10.1016/j.xpro.2024.103256

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Extracellular vesicles (EVs) are secreted, cell-derived, membrane-bound compartments implicated in various diseases for their ability to influence distant targets and as carriers of biomarkers. Here, we present a protocol for separating EVs from mammalian pancreatic cancer cells and their characterization using western blot and electron microscopy. We then demonstrate how they are utilized to affect tumor development in a murine model of metastatic pancreatic cancer including a method to quantify hepatic tumor burden in histologic samples. For complete details on the use and execution of this protocol, please refer to Dudgeon et al.1 1

引用

页数：16

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