The PbbHLH62/PbVHA-B1 module confers salt tolerance through modulating intracellular Na plus /K plus homeostasis and reactive oxygen species removal in pear

被引：1

作者：

Qiao, Qinghai ^{[1
,3
]}

Huang, Yongdan ^{[1
,2
]}

Dong, Huizhen ^{[1
,2
]}

Xing, Caihua ^{[1
,2
]}

Han, Chenyang ^{[1
,2
]}

Lin, Likun ^{[1
,2
]}

Wang, Xin ^{[1
,2
]}

Su, Zhiyuan ^{[1
,2
]}

Qi, Kaijie ^{[1
,2
]}

Xie, Zhihua ^{[1
,2
]}

Huang, Xiaosan ^{[1
,2
]}

Zhang, Shaoling ^{[1
,2
,3
]}

机构：

[1] Nanjing Agr Univ, State Key Lab Crop Genet & Germplasm Enhancement &, Nanjing 210095, Peoples R China

[2] Nanjing Agr Univ, Coll Hort, Nanjing 210095, Peoples R China

[3] Nanjing Agr Univ, Coll Life Sci, Nanjing 210095, Peoples R China

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2024年 / 210卷

基金：

美国国家科学基金会;

关键词：

V-ATPase; PbVHA-B1; PbbHLH62; Salt tolerance; Ion and reactive oxygen species homeostasis; P.betulaefolia; VACUOLAR H+-ATPASE; HELIX TRANSCRIPTION FACTOR; OVERLY SENSITIVE PATHWAY; PLASMA-MEMBRANE; STRESS TOLERANCE; ION HOMEOSTASIS; DIFFERENTIAL EXPRESSION; ARABIDOPSIS-THALIANA; GENE-EXPRESSION; NA+/H+ EXCHANGE;

D O I：

10.1016/j.plaphy.2024.108663

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The vacuolar H + -ATPase (V-ATPase) is a multi -subunit membrane protein complex, which plays pivotal roles in building up an electrochemical H + -gradient across tonoplast, energizing Na + sequestration into the central vacuole, and enhancing salt stress tolerance in plants. In this study, a B subunit of V-ATPase gene, PbVHA-B1 was discovered and isolated from stress -induced P . betulaefolia combining with RT-PCR method. The RT-qPCR analysis revealed that the expression level of PbVHA-B1 was upregulated by salt, drought, cold, and exogenous ABA treatment. Subcellular localization analyses showed that PbVHA-B1 was located in the cytoplasm and nucleus. Moreover, overexpression of PbVHA-B1 gene noticeably increased the ATPase activity and the tolerance to salt in transgenic Arabidopsis plants. In contrast, knockdown of PbVHA-B1 gene in P . betulaefolia by virusinduced gene silencing had reduced resistance to salt stress. In addition, using yeast one-hybride (Y1H) and yeast two-hybride (Y2H) screens, PbbHLH62, a bHLH transcription factor, was identified as a partner of the PbVHA-B1 promoter and protein. Then, we also found that PbbHLH62 positively regulate the expression of PbVHA-B1 and the ATPase activity after salt stress treatment. These findings provide evidence that PbbHLH62 played a critical role in the salt response. Collectively, our results demonstrate that a PbbHLH62/PbVHA-B1 module plays a positive role in salt tolerance by maintain intracellular ion and ROS homeostasis in pear.

引用

页数：16