Highly Biocompatible Hemoglobin-Stabilized Gold Nanoparticles for an Enhanced Catalytic Reduction of 4-Nitrophenol

4-nitrophenol (4-NP) is a frequently encountered toxic phenolic organic pollutant in water. It is important to develop a simple method to treat 4-NP. Small and monodispersed gold nanoparticles often have good catalytic performance of 4-NP. Hemoglobin (Hb) is a kind of common and important protein in organisms. Herein, highly biocompatible bovine hemoglobin-stabilized gold nanoparticles (Aun-Hb NPs) were synthesized using hemoglobin as a biological template. Then, the size, zeta potential, and composition of Aun-Hb NPs were investigated by transmission electron microscopy, dynamic light scattering, and X-ray photoelectron spectroscopy. The Aun-Hb NPs with small gold nanoparticles of about 1.4-2.4 nm had good catalytic capabilities in reducing 4-NP to form 4-aminophenol. Au20-Hb NPs demonstrated superior catalytic efficiency in the reduction of 4-NP when compared to other nanoparticles. Moreover, as-synthesized Au20-Hb NPs exhibited excellent biocompatibility through the MTT experiment. The method of preparation of gold nanoparticles offers one way to prepare metal nanoparticles for good potential catalytic applications of gold nanoparticles.