FluoAnalysis: An Open-Source MATLAB Toolbox for Analysis of Calcium Imaging Measurements of Oscillatory Astrocytic and Neuronal Networks

被引:0
|
作者
Peter, Marton [1 ,2 ]
Heja, Laszlo [1 ]
机构
[1] HUN REN Res Ctr Nat Sci, Inst Organ Chem, Magyar Tudosok korutja 2, H-1117 Budapest, Hungary
[2] Eotvos Lorand Univ, Hevesy Gyorgy PhD Sch Chem, H-1117 Budapest, Hungary
关键词
astrocytes; neurons; neural activity; software; two-photon; electrophysiology; oscillations; spectral analysis; DECONVOLUTION;
D O I
10.3390/brainsci14080830
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Calcium imaging, especially two-photon imaging, has become essential in neuroscience for studying neuronal and astrocytic activity under in vivo and in vitro conditions. Current advances in the development of calcium sensors as well as imaging hardware enable high-frequency measurements of calcium signals in hundreds of cells simultaneously. The analysis of these large datasets requires special tools and usually a certain level of programming experience. Despite advancements in calcium imaging analysis software development, significant gaps remain, particularly for data acquired at a high sampling rate that would allow for the spectral analysis of calcium signals. The FluoAnalysis MATLAB toolbox addresses these gaps by offering a comprehensive solution for analyzing simultaneously measured calcium imaging and electrophysiological data. It features both GUI-based and command-line approaches, emphasizing frequency domain analysis to reveal network-level oscillatory signals linked to single-cell activity. In addition, the toolbox puts special emphasis on differentiating between astrocytes and neurons, revealing the interactions between the network activity of the two major cell types of the brain. It facilitates a streamlined workflow for data loading, ROI identification, cell classification, fluorescence intensity calculation, spectral analysis, and report generation, supporting both manual and automated high-throughput analysis. This versatile platform enables the comprehensive analysis of large imaging datasets. In conclusion, the FluoAnalysis MATLAB toolbox provides a robust and versatile platform for the integrated analysis of calcium imaging and electrophysiological data, supporting diverse neuroscience research applications.
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页数:11
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