Anticancer effect of Poly(N-vinyl pyrrolidone/chitosan/silver nanoparticles) nanocomposite synthesized by gamma radiation

被引:0
|
作者
Ali Hassan, Mohamed [1 ]
El-Deap, Mohamed [2 ]
Mahmoud Farag, Ahmed [2 ]
Abdelrahman, Ibrahim Y. [3 ]
Abdel-Ghaffar, Ashraf Maher [1 ,4 ]
机构
[1] Egyptian Atom Energy Author, Natl Ctr Radiat Res & Technol, Radiat Res Polymer Chem Dept, Cairo, Egypt
[2] Cairo Univ, Chem Dept, Giza, Egypt
[3] Egyptian Atom Energy Author, Natl Ctr Radiat Res & Technol, Radiat Biol Dept, Cairo, Egypt
[4] Egyptian Atom Energy Author, Natl Ctr Radiat Res & Technol, Radiat Res Polymer Chem Dept, Ind Irradiat Div, 3 Ahmed El Zomor St, Cairo 29, Egypt
关键词
Gamma radiation; poly(NVP/CS/AgNPs) nanocomposite; silver nanoparticles; cytotoxicity; anticancer; SILVER NANOPARTICLES; ANTIOXIDANT;
D O I
10.1177/08927057241259757
中图分类号
TB33 [复合材料];
学科分类号
摘要
Poly(N-vinyl pyrrolidone/chitosan/Ag nanoparticles) Poly (NVP/CS/AgNPs) nanocomposite was prepared by using gamma radiation. Silver nitrate was reduced to AgNPs by using gamma radiation to create highly stable, and evenly dispersed AgNPs within the nanocomposite network. Fourier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), transmission electron microscopy (TEM), and scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM-EDX) analysis are used to identify the characteristics of the prepared nanocomposite. The newly biocompatible Poly (NVP/CS/AgNPs) nanocomposite prepared by the gamma radiation is used as a model for an anticancer drug. Regarding its application on cancer cells, the results of the outcome demonstrate that the Poly (NVP/CS/AgNPs) nanocomposite significantly decreased the HepG2 cell viability from 100% to 1.5% after 72 hours using the concentration of 30 mu g/ml, and the IC50 recorded 10.024 mu g/ml at 72 hours of treatment. Moreover, the treatment with the Poly (NVP/CS/AgNPs) nanocomposite increases the cytotoxicity against cancer cells at different concentrations 30 mu g/ml and 3.75 mu g/ml at different time intervals of 24, 48, and 72 hours. The percent change of MDA, and H2O2 increased in treated cells, and was recorded at 127%, and 69%, respectively, but the CAT activity decreased by 47% in comparison with untreated cells. Relative gene expression was performed in the control, and treated group. The gene expression revealed that the Bcl-2 gene expression as the anti-apoptotic gene was downregulated in the treated group. In contrast, the apoptotic gene BAX was upregulated, and reached 7383-fold in the group treated. The Bax/Bcl2 ratio reached 625.19 which is an excellent indicator of apoptosis. Using western blot, the protein expression increased for Caspse-3 and P53 recorded at 203% and 109%, respectively. In conclusion, Poly (NVP/CS/AgNPs) nanocomposite has a potent effect on killing cancer cells.
引用
收藏
页码:1018 / 1039
页数:22
相关论文
共 50 条
  • [1] In Vivo Study of Silver Nanoparticles Entrapped Poly(N-vinyl pyrrolidone/Dextran) Hydrogel Synthesized by Gamma Radiation on the Antitumor Activity of Doxorubicin
    El-Arnaouty, M. B.
    Eid, M.
    Mansour, H. H.
    JOURNAL OF INORGANIC AND ORGANOMETALLIC POLYMERS AND MATERIALS, 2021, 31 (06) : 2700 - 2710
  • [2] In Vivo Study of Silver Nanoparticles Entrapped Poly(N-vinyl pyrrolidone/Dextran) Hydrogel Synthesized by Gamma Radiation on the Antitumor Activity of Doxorubicin
    M. B. El-Arnaouty
    M. Eid
    H. H. Mansour
    Journal of Inorganic and Organometallic Polymers and Materials, 2021, 31 : 2700 - 2710
  • [3] TACTICITY OF POLY(N-VINYL PYRROLIDONE)
    CHENG, HN
    SMITH, TE
    VITUS, DM
    JOURNAL OF POLYMER SCIENCE PART C-POLYMER LETTERS, 1981, 19 (01) : 29 - 31
  • [4] In-situ formation of silver nanoparticles on poly (lactic acid) film by γ-radiation induced grafting of N-vinyl pyrrolidone
    Wang, Jingxia
    Chen, Hao
    Chen, Zhuping
    Chen, Yuheng
    Guo, Dan
    Ni, Maojun
    Liu, Siyang
    Peng, Chaorong
    MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS, 2016, 63 : 142 - 149
  • [5] Glass transition temperature of chitosan and miscibility of chitosan/poly(N-vinyl pyrrolidone) blends
    Sakurai, K
    Maegawa, T
    Takahashi, T
    POLYMER, 2000, 41 (19) : 7051 - 7056
  • [6] Radiation synthesis of poly(N-vinyl pyrrolidone\chitosan\itaconic acid\ZnO) nanocomposite hydrogel for antimicrobial activity and controlled release of amoxicillin
    Fadl, Faten I. Abou El
    Ghaffar, Ashraf M. Abdel
    El-Sawy, Naeem M.
    POLYMERS & POLYMER COMPOSITES, 2022, 30
  • [7] Radiation synthesis of poly(N-vinyl pyrrolidone\chitosan\itaconic acid\ZnO) nanocomposite hydrogel for antimicrobial activity and controlled release of amoxicillin
    Abou El Fadl, Faten I.
    Abdel Ghaffar, Ashraf M.
    El-Sawy, Naeem M.
    POLYMERS & POLYMER COMPOSITES, 2022, 30
  • [8] Medicated wound dressings based on poly(vinyl alcohol)/poly(N-vinyl pyrrolidone)/chitosan hydrogels
    Yu, Haijun
    Xu, Xiaoyi
    Chen, Xuesi
    Hao, Jingquan
    Jing, Xiabin
    Journal of Applied Polymer Science, 2006, 101 (04): : 2453 - 2463
  • [9] Medicated wound dressings based on poly(vinyl alcohol)/poly(N-vinyl pyrrolidone)/chitosan hydrogels
    Yu, Haijun
    Xu, Xiaoyi
    Chen, Xuesi
    Hao, Jingquan
    Jing, Xiabin
    JOURNAL OF APPLIED POLYMER SCIENCE, 2006, 101 (04) : 2453 - 2463
  • [10] Immobilization of Lipase on Poly(N-Vinyl Pyrrolidone)
    T. A. Kovaleva
    A. S. Belenova
    G. V. Shatalov
    Bulletin of Experimental Biology and Medicine, 2011, 151 : 415 - 417