Intelligent near-infrared light-activatable DNA machine with DNA wire nano-scaffold-integrated fast domino-like driving amplification for high-performance imaging in live biological samples

被引：2

作者：

Zhang, Tiantian ^{[1
]}

Sun, Xiaoming ^{[2
]}

Chen, Xiaoxue ^{[1
]}

Chen, Weilin ^{[1
]}

Tang, Hongwu ^{[3
]}

Li, Cheng-Yu ^{[1
]}

机构：

[1] Wuhan Univ Sci & Technol, Sch Publ Hlth, Wuhan 430065, Peoples R China

[2] Hubei Univ Med, Biomed Res Inst, Sch Basic Med Sci, Hubei Key Lab Embryon Stem Cell Res, Shiyan 442000, Peoples R China

[3] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2024年 / 259卷

基金：

中国国家自然科学基金;

关键词：

Near -infrared light; DNA machine; DNA wire nano -scaffold; Domino -like driving; Imaging; Live biological samples; LIVING CELLS; IN-VITRO; CANCER; DRIVEN;

D O I：

10.1016/j.bios.2024.116412

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

While there is significant potential for DNA machine-built enzyme-free fluorescence biosensors in the imaging analysis of live biological samples, they persist certain shortcomings. These encompass a deficiency of signal enrichment within a singular interface, uncontrolled premature activation during bio-delivery, and a slow reaction rate due to free nucleic acid collisions. In this contribution, we are committed to resolving the above challenges. Firstly, a single-interface-integrated domino-like driving amplification is constructed. In this conception, a specific target acts as the domino promotor (namely the energy source), initiating a cascading chain reaction that grafts onto a singular interface. Next, an 808 nm near-infrared (NIR) light-excited up-converting luminescence-induced light-activatable biosensing technique is introduced. By locking the target-specific identification segment with a photo-cleavage connector, the up-converted ultraviolet emission can activate target binding in a completely controlled manner. Moreover, a fast reaction rate is achieved by confining nucleic acid collisions within the surface of a DNA wire nano-scaffold, leading to a substantial enhancement in local contact concentration (30.8-fold increase, alongside a 15 times elevation in rate). When a non-coding microRNA (miRNA-221) is positioned as the model low-abundance target for proof-of-concept validation, our intelligent DNA machine demonstrates ultra-high sensitivity (with a limit of detection down to 62.65 fM) and good specificity for this hepatic malignant tumor-associated biomarker in solution detection. Going further, it is worth highlighting that the biosensing system can be employed to carry out high-performance imaging analysis in live bio-samples (ranging from the cellular level to the nude mouse body), thereby propelling the field of DNA machines in disease diagnosis.

引用

页数：11