Design and research of fluorescence microscopic imaging detection device

In recent years, the incidence of allergic diseases is getting higher and higher, and there is almost no radical cure. However, early detection of allergens is very important for preventing and avoiding allergies, and allergen detection technology has become a popular research object. This paper describes a novel allergen detection device that combines optical, mechanical and electronic techniques. The device is based on quantum dots, a fluorescent marker, which is combined with the quantum dots and interpreted according to the fluorescence intensity value of the quantum dots excited by the collection. The device is composed of image acquisition module, motion module and control module. Ultraviolet LED lights are used as light sources. The mechanical characteristics of moving components are determined by finite element analysis. The S-type acceleration control provides balanced and accurate conditions for image acquisition. In the optical section, the lighting path is simulated to ensure even light on the target surface, while the lens design is also simulated and optimized to ensure clear image acquisition. After image acquisition, initial processing is performed, including image correction, speckle noise reduction, and then the fluorescence brightness of the image is analyzed. The device has been simulated by finite element analysis, and its moving parts meet the design requirements. The operating error of the motor is within the control range, and the lighting light and lens design have also reached the design standard after Zmax simulation test. The fluorescence intensity of the fluorescence image is balanced, and the error is less than 3%. The design of the device provides a foundation for the molecular diagnosis industry.