Co-immobilization of a bi-enzymatic cascade into hierarchically porous MIL-53 for efficient 6′-sialyllactose production

被引：4

作者：

Peng, Xinlv ^{[1
,2
,3
]}

Pei, Caixia ^{[1
,2
,4
]}

Qian, Eika W. ^{[4
]}

Du, Yuguang ^{[1
,2
]}

Li, Jian-Jun ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Proc Engn, State Key Lab Biochem Engn, Beijing 100190, Peoples R China

[2] Chinese Acad Sci, Key Lab Biopharmaceut Preparat & Delivery, Beijing 100190, Peoples R China

[3] Univ Chinese Acad Sci, Sch Chem Engn, Beijing 100049, Peoples R China

[4] Tokyo Univ Agr & Technol, Grad Sch Bioapplicat & Syst Engn, Nakacho 2-24-16, Koganei, Tokyo 1848588, Japan

来源：

NANOSCALE | 2024年 / 16卷 / 31期

关键词：

HUMAN-MILK OLIGOSACCHARIDES; METAL-ORGANIC FRAMEWORKS; CHEMOENZYMATIC SYNTHESIS; STRATEGY; SYSTEM; YIELD; MOF;

D O I：

10.1039/d4nr01775g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

6 '-Sialyllactose (6 '-SL), the most abundant sialylated human milk oligosaccharide, has attracted attention for its potential application in supplementary infant formulas. Herein, we report a facile strategy to construct a cascade bioreactor for the enzymatic synthesis of 6 '-SL by co-immobilizing an enzymatic module consisting of CMP-sialic acid synthase and alpha-2,6-sialyltransferase into hierarchically porous MIL-53 (HP-MIL-53). The as-prepared HP-MIL-53 showed high enzyme immobilization capacity, reaching 226 mg g(-1). Furthermore, the co-immobilized enzymes exhibited higher initial catalytic efficiency, and thermal, pH and storage stability than the free ones. Finally, the 6 '-SL yield remained >80% after 13 cycles of use. We expect that HP-MIL-53 would have potential industrial applications in the enzymatic modular synthesis of 6 '-SL and other glycans.

引用

页码：14932 / 14939

页数：8