Regulatory roles of miRNA-530 in the post-transcriptional regulation of NF-KB signaling pathway through targeted modulation of IκBα in Sebastes schlegelii

MicroRNAs (miRNAs) are a crucial type of non-coding RNAs involved in post-transcriptional regulation. The playing essential regulatory roles in the NF -KB signaling pathway and modulate the host immune response to diverse pathogens by targeting I kappa B alpha. However, the regulatory mechanism of miRNAs in relation with I kappa B alpha in Sebastes schlegelii remains unclear. In our study, we identified two copies of IkB alpha gene in black rockfish (Sebastes schlegelii), namely IkB alpha 1 and IkB alpha 2. Moreover, we have discovered that miRNA-530 can activate the NF -KB signaling pathway by inhibiting the expression of I kappa B alpha, thereby inducing the inflammatory response. This project comprehensively investigated the interactive regulatory roles of miRNA-530 in the NF -KB signaling pathway at both cellular and in vivo levels, while also elucidating the regulatory relationships between miRNA-530 and I kappa B alpha. In conclusion, our research confirmed that miRNA-530 can target the 3 ' UTR region of I kappa B alpha, resulting in a decrease in the expression of I kappa B alpha at the post-transcriptional level and inhibiting its translation. The findings contribute to the understanding of the regulatory network of non-coding RNA in teleosts and its subsequent regulation of the NF -KB signaling pathway by miRNAs.