Silver-doped glass-ceramic scaffolds with antibacterial and bioactive properties for bone substitution

The preparation of scaffolds that are both macroporous and mechanically strong is a significant challenge in the development of bioactive ceramic materials for bone substitution. Furthermore, the introduction of beneficial extra-functionalities such as bacterial inhibition is highly appealing but adds complexity to implant design and production. In this study, we aimed to fabricate highly porous, bioactive and antibacterial glass-ceramic scaffolds with interconnected macropores through the foam replica method. The scaffolds were sintered at two different temperatures (620 and 850 degrees C), yielding glassy or partially crystallized materials, respectively. The scaffold produced at higher temperature was found to be highly porous (>75 vol%), mechanically stronger and able to induce hydroxyapatite formation after three days of soaking in SBF (in vitro bioactivity). In order to confer antibacterial activity, silver (Ag) ions were introduced onto the scaffold surface through ion exchange in an aqueous solution. Compositional analysis confirmed the successful doping of the scaffold surface with silver, which was continuously released in SBF for at least 28 days, as revealed by ICP-MS. Finally, the antibacterial action of the Ag-doped scaffold was confirmed towards Staphylococcus epidermidis. Overall, the results reported in this work show the potential of foam-like Ag-doped bioactive glass-ceramic scaffolds to be used in applications requiring bone substitution and antibacterial properties.