Developing biomass augmentation strategy for cultivation of Marvania coccoides using fruit waste and wastewater based growth medium for biodiesel production

Microalgae cultivation using waste as nutrient source can minimize the use of expensive chemical nutrients and valuable freshwater. In present work, novel microalgae Marvania coccoides was cultivated in fruit waste (FW) and wastewater (WW) based growth medium. To further augment metabolites and biomass, the culture was supplemented with phytohormone, kinetin (K). Various pre-treatment methods were investigated for improving the nutrient release and bacterial load reduction in waste-based medium. Phytohormone supplemented fruit waste and wastewater media (WW + FW + K) showed improved biomass productivity of 117.14 mg.L-1.d-1 compared to both waste-based and synthetic medium. Biomass harvested from WW + FW + K showed increased lipid content (22 %) as compared to lipid content (19 %) of biomass from synthetic medium. Biodiesel yield of 91.50 % was observed with fatty acids C16:0, C16:2, C18:0, C18:1, C18:2, C19:0, C20:1, C20:2 and C22:1 in composition. M. coccoides can be cultivated in waste medium and used as promising feedstock for production of biodiesel.