Comparison of two different device results measuring HbA1c by high performance liquid chromatography (HPLC) method

Aim: HbA1c results, which are routinely measured in HA-8180T and HA-8180V model devices in our laboratory for HbA1c measurement, were divided into three groups based on diabetes mellitus diagnostic criteria with the aim of investigating the compatibility between the two devices at different HbA1c concentrations. Material and Methods: For HbA1c analysis, the HbA1c values of 260 patients were measured in two devices (Arkray Adams HA-8180T and Arkray Adams HA-8180V) using the ion exchange chromatography method. According to the measured % HbA1c values, 3 groups (1st Group; n=87<5.7%, 2nd Group; n=96 5.7%- 6.4% and 3rd Group; n=77> 6.5%). Results: Correlation (r=0.994, 95% Confidence Interval (CI) = 0.993-0.996, p<0.0001) of the measurement results obtained between the two devices and Passing-Bablok regression analysis [HA-8180T = 1.0xHA-8180V-0.20] (Slope 95% CI= 1.0-1.0, intercept 95% CI: -0.20-0.20) equation were obtained. According to the regression equation, the linearity between the devices was found to be (cusum test; p=0.90). In the Bland-Altman plot to evaluate the compatibility of the two devices, it was observed that the percentage change between the %HbA1c results obtained with HA-8180-T and the %HbA1c results obtained with HA-8180-V was 3% (95% CI: 2.83 - 3.12) higher on average. Discussion: Due to the compatibility of the results measured between the two devices in this study, we think that the use of the HA-8180V device, which has a shorter result time, in laboratories with a higher number of tests may be appropriate in terms of reducing the workload.