Investigation of the affinity and interaction of fibrinogen with trehalose as a protein stabilizer

被引:3
|
作者
Khoshkalam, Kasra [1 ]
Izadi, Zhila [2 ,3 ]
Mirhaji, Samaneh Sadat [4 ]
Soleimanpour, Marjan [4 ]
Ghasemi, Mina Darabi [2 ,3 ]
Barzegari, Ebrahim [5 ]
Jaymand, Mehdi [6 ]
Lotfabadi, Alireza [2 ,3 ]
Derakhshankhah, Hossein [2 ,3 ]
Saboury, Ali Akbar [4 ]
Rautray, Tapash Ranjan [7 ]
机构
[1] Kermanshah Univ Med Sci, Student Res Comm, Kermanshah, Iran
[2] Kermanshah Univ Med Sci, Hlth Inst, Pharmaceut Sci Res Ctr, Kermanshah, Iran
[3] Kermanshah Univ Med Sci, USERN Off, Kermanshah, Iran
[4] Univ Tehran, Inst Biochem & Biophys, Tehran, Iran
[5] Kermanshah Univ Med Sci, Hlth Technol Inst, Med Biol Res Ctr, Kermanshah, Iran
[6] Kermanshah Univ Med Sci, Hlth Technol Inst, Nano Drug Delivery Res Ctr, Kermanshah, Iran
[7] Siksha O Anusandhan, Inst Tech Educ & Res, Ctr Excellence TM Sci, Biomat & Tissue Regenerat Lab, Bhubaneswar, Odisha, India
关键词
Fibrinogen; Trehalose; Protein structure stabilizer; Ligand-protein; Cytotoxicity; QUANTITATIVE IR SPECTROPHOTOMETRY; WATER H2O SOLUTIONS; FLUORESCENCE SPECTROSCOPY; PEPTIDE COMPOUNDS; CRYSTAL-STRUCTURE; CONCENTRATE; PARAMETERS;
D O I
10.1016/j.molliq.2024.124713
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
Trehalose, a remarkable substance, holds great importance in biomedicine for its anti-inflammatory properties and its capacity to hinder scar formation at wounds. This study aims to explore how trehalose interacts with fibrinogen, a crucial protein in wound healing, paying particular attention to their structural characteristics. An array of analytical methods, such as steady-state fluorescence, UV-Vis analysis, circular dichroism (CD), Zeta potential measurements, Dynamic light scattering (DLS), and Fourier-transform infrared spectroscopy (FT-IR), are employed to examine the influence of trehalose on fibrinogen. The findings demonstrate that trehalose induces structural alterations on the surface of fibrinogen, resulting in its compaction. Moreover, computational modeling approaches like docking and molecular dynamics simulations are utilized to enhance the understanding of the interactions between trehalose and fibrinogen. The findings indicate the establishment of a stable complex between the two entities, accompanied by slight modifications in the protein's structure due to their interaction. The main forces facilitating the bonding of trehalose (the ligand) and the protein are hydrogen bonds and van der Waals forces, resulting in an entropy-driven spontaneous bonding process. FT-IR data reveals the emergence of fresh bonds between fibrinogen and trehalose post their interaction, whereas Zeta potential investigations suggest that the engagement with trehalose enhances the stability of the protein structure. Tests assessing cytotoxicity on a typical fibroblast cell line demonstrated that the fibrinogen-trehalose compound displays lower toxicity levels compared to trehalose alone. This suggests a milder impact on cells from the compound. Moreover, molecular docking analysis supports these experimental results, indicating that trehalose molecules primarily bind to the C-terminal end of the coiled-coil segment and the globular area of the B beta chain within the D beta fragment. These outcomes highlight the promise of trehalose as a valuable material for wound dressing purposes.
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页数:9
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