Structural Design of Full-automatic Pathological Staining System Based on FISH Technology

被引：0

作者：

Lin C. ^{[1
]}

Gan L. ^{[1
]}

Tan X. ^{[1
]}

Li X. ^{[2
]}

Yan W. ^{[2
]}

Li Q. ^{[2
]}

Chen G. ^{[2
]}

Liao G. ^{[1
]}

Liu Z. ^{[1
]}

机构：

[1] School of Mechanical Science and Engineering, Huazhong University of Science and Technology, Wuhan

[2] Wuhan Health Care Biotechnology Co.,Ltd., Wuhan

来源：

Zhongguo Jixie Gongcheng/China Mechanical Engineering | 2023年 / 34卷 / 05期

关键词：

fixture module; fluorescence in situ hybridization(FISH); full-automatic pathological staining system; mechanical structure; motion module;

D O I：

10.3969/j.issn.1004-132X.2023.05.011

中图分类号：

学科分类号：

摘要：

FISH technology was a common method to detect the nucleic acid sequence of malignant tumor cells. It was widely used in the field of cancer diagnoses and treatments. In order to realize the full automation of FISH experiments, a full-automatic pathological staining system was designed, which was provided with multi axis control arm, reagent sampler, slide, cover slide fixture, auxiliary module and so on. The positioning erorrs of the multi axis control arm are less than ±0.1 mm, the accuracy of extracting and discarding the pipette head is more than 99.5%, the success rate of extracting the cover glass is more than 99%, and the erorrs of micro reagent sampling are less than 0.6 μL. The errors of adding a large amount of reagent are less than 0.5 mL, the temperature control erorrs of slide clamp are less than ±1 °C, and there is no reagent leakage. The results of biological experiments show that the system has good fluorescence staining effectiveness, the signal points are clearly visible, and the interpretable rate of fluorescence image is more than 90%. © 2023 China Mechanical Engineering Magazine Office. All rights reserved.

引用

页码：603 / 609

页数：6

共 13 条

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