Clostridium butyricum Argonaute-powered programmable platform using a magnetic nanoparticle tetrahedral DNA encoding system for the ultrasensitive and multiplexed detection of non-nucleic acid targets

被引：7

作者：

Li, Letian ^{[2
]}

Wang, Mengjiao ^{[2
]}

Wen, Junping ^{[2
]}

Hong, Feng ^{[2
]}

Ren, Liangqiong ^{[2
]}

Jiang, Feng ^{[3
]}

Chen, Yiping ^{[1
,2
]}

机构：

[1] Dalian Polytech Univ, State Key Lab Marine Food Proc & Safety Control, Dalian 116034, Liaoning, Peoples R China

[2] Huazhong Agr Univ, Coll Food Sci & Technol, Wuhan 430070, Peoples R China

[3] Key Lab Detect Technol Focus Chem Hazards Anim Der, Wuhan 430075, Peoples R China

来源：

CHEMICAL ENGINEERING JOURNAL | 2024年 / 484卷

基金：

中国国家自然科学基金;

关键词：

Clostridium butyricum Argonaute; Tetrahedral DNA encoding; Non-nucleic acid target; Multiple detection; SURFACE-CHARGE DENSITY; PYROCOCCUS-FURIOSUS; AMPLIFICATION; CRISPR-CAS12A;

D O I：

10.1016/j.cej.2024.149548

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Multiplexed detection of hazardous factors is important for food safety and public health. Argonaute (Ago) has been proposed as a promising multiplexed detection tool for its ability to specifically recognize and cleave target sequences. However, most reported thermophilic Ago-based sensors require high reaction temperatures and have limited applications for non-nucleic acid target detection. To overcome the temperature limitations and extend the application of Ago to multiplexed non-nucleic acid target detection, we herein report a mesophilic Clostridium butyricum Argonaute (CbAgo)-Powered Programmable (APP) platform combined with a magnetic nanoparticle tetrahedral DNA encoding system to quantitatively detect multiple non-nucleic acid targets at 37 C. This platform achieved satisfactory results in the multiplexed detection of three mycotoxins in 30 food samples (pg/ mL level) and three pathogens in 100 clinical samples (102 CFU/mL level without DNA extraction), highlighting its potential as a multiplexed and ultrasensitive detection tool in food safety, clinical diagnosis, and so forth.

引用

页数：13

共 1 条

[1] Mesophilic Argonaute-Based Single Polystyrene Sphere Aptamer Fluorescence Platform for the Multiplexed and Ultrasensitive Detection of Non-Nucleic Acid Targets
Lu, Yingying
Wen, Junping
Wang, Chengming
Wang, Mengjiao
Jiang, Feng
Miao, Lin
Xu, Minggao
Li, Yingjun
Chen, Xiaohua
Chen, Yiping
SMALL, 2024, 20 (20)

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