MiRNA-27a通过PPARγ介导3T3-L1细胞胰岛素抵抗

被引：2
|
作者：
谢军
韩造木
机构：
[1] 华中科技大学同济医学院附属武汉中心医院后湖综合科
来源：
华南国防医学杂志 | 2017年 / 31卷 / 05期
关键词：
MiRNA-27a; 胰岛素抵抗; 3T3-L1脂肪细胞; 过氧化物酶体增殖物激活受体γ;
D O I：
暂无
中图分类号：
R587.1 [糖尿病];
学科分类号：
摘要：
目的初步探讨miRNA-27a介导3T3-L1细胞胰岛素抵抗的机制。方法通过肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)诱导建立3T3-L1脂肪细胞胰岛素抵抗的细胞模型,观察miRNA-27a在3T3-L1细胞胰岛素抵抗模型中的表达变化,逆转录聚合酶链式反应(reverse transcription-polymerase chain reaction,RT-PCR)检测miRNA-27a对过氧化物酶体增殖物激活受体γ(peroxisome proliferator activated receptor-gamma,PPARγ)mRNA的调控作用。结果miRNA-27a在3T3-L1细胞胰岛素抵抗模型中表达上调,miRNA-27a模拟物抑制PPARγmRNA的表达,miRNA-27a抑制剂上调PPARγmRNA的表达。结论 miRNA-27a介导的3T3-L1细胞胰岛素抵抗可能是通过靶向PPARγ实现。
引用
收藏
页码：293 / 296
页数：4
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