Expression of putative zinc-finger protein lcn61 gene in lymphocystis disease virus China (LCDV-cn) genome

被引：0
|
作者：
闫秀英 [1 ]
孙修勤 [2 ]
机构：
[1] College of Llife Science and Technology,Ocean University of China
[2] First Institute of
来源：
Chinese Journal of Oceanology and Limnology | 2009年 / 27卷 / 02期
关键词：
D O I：
暂无
中图分类号：
Q939.4 [病毒（滤过性病毒）];
学科分类号：
摘要：
An open reading frame (lcn61) of lymphocystis disease virus China (LCDV-cn), probably responsible for encoding putative zinc-finger proteins was amplified and inserted into pET24a (+) vector. Then it expressed in E. coli BL21 (DE3), and His-tag fusion protein of high yield was obtained. It was found that the fusion protein existed in E. coli mainly as inclusion bodies. The bioinformatics analysis indicates that LCN61 is C2H2 type zinc-finger protein containing four C2H2 zinc-finger motifs. This work provides a theory for functional research of lcn61 gene.
引用
收藏
页码：337 / 341
页数：5
相关论文
共 7 条
1