RAPID AUTOMATED QUANTITATION OF ISOLEUCINE, LEUCINE, TYROSINE AND PHENYLALANINE FROM DRIED BLOOD FILTER-PAPER SPECIMENS

被引：19
|
作者：
QU, Y
MILLER, JB
SLOCUM, RH
SHAPIRA, E
机构：
[1] TULANE UNIV,SCH MED,HUMAN GENET PROGRAM,1430 TULANE AVE,NEW ORLEANS,LA 70112
[2] BECKMAN INSTRUMENTS INC,RES DEPT,FULLERTON,CA 92634
来源：
CLINICA CHIMICA ACTA | 1991年 / 203卷 / 2-3期
关键词：
PHENYLKETONURIA (PKU); MAPLE SYRUP URINE DISEASE (MSUD); TYROSINEMIA; NEWBORN SCREENING; HPLC;
D O I：
10.1016/0009-8981(91)90291-J
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Isothermal and isocratic HPLC chromatography for rapid quantitation of isoleucine and leucine (merged peak), tyrosine and phenylalanine from a single dried blood filter paper specimen (1/8" dot blot) is described. The net chromatography time of the relevant peaks is less than 3 min. The results can be used to screen for PKU, MSUD, and tyrosinemia in one single assay. The advantage for early determination of PKU by simultaneous quantitation of both phenylalanine and tyrosine with the determination of their ratio is discussed. Using a dual column system this procedure might be suitable for large-scale newborn screening.
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页码：191 / 198
页数：8
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