SPECIFIC INSULIN-LIKE GROWTH FACTOR-I RECEPTORS ON CIRCULATING BOVINE MONONUCLEAR-CELLS

被引：18
|
作者：
ZHAO, X
MCBRIDE, BW
TROUTENRADFORD, LM
BURTON, JH
机构：
[1] Department of Animal and Poultry Science, University of Guelph Guelph, ON
来源：
JOURNAL OF RECEPTOR RESEARCH | 1992年 / 12卷 / 01期
关键词：
D O I：
10.3109/10799899209066027
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
We have examined the presence and properties of specific receptors for IGF-I on bovine mononuclear cells. Competitive binding studies showed that binding of [I-125]IGF-I to mononuclear cells was inhibited by unlabelled peptides with the rank of IGF-I > IGF-II > insulin. The binding of [I-125]IGF-I was a function of the cell concentration. Equilibrium dissociation constant and receptor concentration values for the average of 9 adult cows were 1.13 +/- 0.11 nM and 108.9 +/- 24.1 fMol/10(7) cells, respectively. Moreover, IGF-I stimulated thymidine incorporation into bovine mononuclear cells in the absence of serum and phytohemagglutinin (PHA). The existence of specific and functional IGF-I receptors on circulating bovine mononuclear cells would provide an easily accessible source for studying IGF-I receptor changes in the bovine, both in physiologic and pathologic states.
引用
收藏
页码：117 / 129
页数：13
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