IDENTIFICATION AND SEQUENCE-ANALYSIS OF A METHYLASE GENE IN PORPHYROMONAS-GINGIVALIS

被引：21

作者：

BANAS, JA ^{[1
]}

FERRETTI, JJ ^{[1
]}

PROGULSKEFOX, A ^{[1
]}

机构：

[1] UNIV FLORIDA,COLL DENT,DEPT ORAL BIOL,GAINESVILLE,FL 32610

来源：

NUCLEIC ACIDS RESEARCH | 1991年 / 19卷 / 15期

关键词：

D O I：

10.1093/nar/19.15.4189

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A gene from the periodontal organism Porphyromonas gingivalis has been identified as encoding a DNA methylase. The gene, referred to as pgiIM, has been sequenced and found to contain a reading frame of 864 basepairs. The putative amino acid sequence of the encoded methylase was 288 amino acids, and shared 47% and 31% homology with the Streptococcus pneumoniae DpnII and E. coli Dam methylases, respectively. The activity and specificity of the pgi methylase (M.PgiI) was confirmed by cloning the gene into a dam- strain of E. coli (JM110) and performing a restriction analysis on the isolated DNA with enzymes whose activities depended upon the methylation state of the DNA. The data indicated that M.Pgil, like DpnII and Dam, methylated the adenine residue within the sequence 5'-GATC-3'.

引用

页码：4189 / 4192

页数：4

共 24 条

[1] A MUTATION IN THE DAM GENE OF VIBRIO-CHOLERAE - 2-AMINOPURINE SENSITIVITY WITH INTACT GATC METHYLASE ACTIVITY [J].

BANDYOPADHYAY, R ;

SENGUPTA, A ;

DAS, J .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 165 (02) :561-567

[2]

BOURGUELERTET L, 1984, NUCLEIC ACIDS RES, V12, P3659

[3] EVIDENCE FOR A METHYLATION-BLOCKING FACTOR (MBF) LOCUS INVOLVED IN PAP PILUS EXPRESSION AND PHASE VARIATION IN ESCHERICHIA-COLI [J].

BRAATEN, BA ;

BLYN, LB ;

SKINNER, BS ;

LOW, DA .

JOURNAL OF BACTERIOLOGY, 1991, 173 (05) :1789-1800

[4] DNA METHYLATION DIFFERENTIALLY ENHANCES THE EXPRESSION OF ONE OF THE 2 ESCHERICHIA-COLI DNAA PROMOTERS INVIVO AND INVITRO [J].

BRAUN, RE ;

WRIGHT, A .

MOLECULAR & GENERAL GENETICS, 1986, 202 (02) :246-250

[5]

BROOKS JE, 1983, NUCLEIC ACIDS RES, V11, P37

[6]

Chandrasegaran S, 1988, STRUCTURE EXPRESSION, V1, P149

[7] COMMON EVOLUTIONARY ORIGIN OF THE PHAGE-T4 DAM AND HOST ESCHERICHIA-COLI DAM DNA-ADENINE METHYLTRANSFERASE GENES [J].

HATTMAN, S ;

WILKINSON, J ;

SWINTON, D ;

SCHLAGMAN, S ;

MACDONALD, PM ;

MOSIG, G .

JOURNAL OF BACTERIOLOGY, 1985, 164 (02) :932-937

[8] PURIFICATION, CLONING AND SEQUENCE-ANALYSIS OF RSRI DNA METHYLTRANSFERASE - LACK OF HOMOLOGY BETWEEN 2 ENZYMES, RSRI AND ECORI, THAT METHYLATE THE SAME NUCLEOTIDE IN IDENTICAL RECOGNITION SEQUENCES [J].

KASZUBSKA, W ;

AIKEN, C ;

OCONNOR, CD ;

GUMPORT, RI .

NUCLEIC ACIDS RESEARCH, 1989, 17 (24) :10403-10425

[9] SEQUENCE MOTIFS CHARACTERISTIC OF DNA[CYTOSINE-N4]METHYLTRANSFERASES - SIMILARITY TO ADENINE AND CYTOSINE-C5 DNA-METHYLASES [J].

KLIMASAUSKAS, S ;

TIMINSKAS, A ;

MENKEVICIUS, S ;

BUTKIENE, D ;

BUTKUS, V ;

JANULAITIS, A .

NUCLEIC ACIDS RESEARCH, 1989, 17 (23) :9823-9832

[10] GATC SEQUENCE AND MISMATCH REPAIR IN ESCHERICHIA-COLI [J].

LAENGLEROUAULT, F ;

MAENHAUTMICHEL, G ;

RADMAN, M .

EMBO JOURNAL, 1986, 5 (08) :2009-2013

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