GLIAL CONDITIONED MEDIUM ALTERS THE EXPRESSION OF AMYLOID PRECURSOR PROTEIN IN SH-SY5Y NEUROBLASTOMA-CELLS

被引：17
|
作者：
HIROSE, Y
IMAI, Y
NAKAJIMA, K
TAKEMOTO, N
TOYA, S
KOHSAKA, S
机构：
[1] NATL INST NEUROSCI,DEPT NEUROCHEM,KODAIRA,TOKYO 187,JAPAN
[2] KEIO UNIV,SCH MED,DEPT NEUROSURG,SHINJU KU,TOKYO 160,JAPAN
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1994年 / 198卷 / 02期
关键词：
D O I：
10.1006/bbrc.1994.1074
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of conditioned medium from astroglia or microglia cultures on the mRNA expression of amyloid precursor protein (APP) in SH-SY5Y human neuroblastoma cells was examined. When incubated with conditioned medium of glial cells, SH-SY5Y cells expressed a larger amount of APP mRNA than the control cells. Reverse transcription followed by the polymerase chain reaction showed marked induction of expression of APP isoforms containing a Kunitz-type protease inhibitor domain. Our results suggest that glial cells may contribute to the regulation of expression of APP in neurons. © 1994 Academic Press, Inc.
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收藏
页码：504 / 509
页数：6
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