CHELATING-AGENTS PROTECT HYDROGENASE AGAINST OXYGEN INACTIVATION

被引：17
作者：
KLIBANOV, AM
KAPLAN, NO
KAMEN, MD
机构：
[1] Department of Chemistry, University of California at San Diego, La Jolla
来源：
BIOCHIMICA ET BIOPHYSICA ACTA | 1979年 / 547卷 / 02期
基金：
美国国家科学基金会;
关键词：
(Clostridium pasteurianum); Chelating agent; Hydrogenase; Oxygen inactivation;
D O I：
10.1016/0005-2728(79)90021-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The effect of chelation on rate of air inactivation of hydrogenase from Clostridium pasteurianum has been investigated. All chelating agents used, whether water-soluble or water-insoluble, afforded protection against oxygen inactivation. EDTA appeared to be the most effective. Thus, in the absence of EDTA, hydrogenase in aqueous solution was nearly totally inactivated after 1 hour incubation in air, whereas 0.5 M EDTA (which did not affect significantly catalytic activity) allowed 41% retention of the initial activity even after 3 days incubation. © 1979.
引用
收藏
页码：411 / 416
页数：6
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