PURIFICATION AND CHARACTERIZATION OF D-2-HALOACID DEHALOGENASE FROM PSEUDOMONAS-PUTIDA STRAIN AJ1/23

被引：54
|
作者：
SMITH, JM
HARRISON, K
COLBY, J
机构：
来源：
JOURNAL OF GENERAL MICROBIOLOGY | 1990年 / 136卷
关键词：
D O I：
10.1099/00221287-136-5-881
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A D-2 haloacid dehalogenase was isolated and purified to homogeneity from Pseudomonas putida strain AJ1/23. The enzyme catalysed the stereospecific dehalogenation of the D-isomer of 2-chloropropionate. Using a new ion-chromatograph assay, the enzyme was found to catalyse the dehalogenation of short-chain 2-halocarboxylic acids. Maximum enzyme activity occurred at pH 9.5 and 50°C and the enzyme was insensitive to most -SH reagents. The enzyme has an M(r) of about 135000 and appears to be composed of four subunits of identical M(r).
引用
收藏
页码：881 / 886
页数：6
相关论文
共 50 条
  • [1] IMMOBILIZATION OF THE D-2-HALOACID DEHALOGENASE FROM PSEUDOMONAS-PUTIDA STRAIN AJ1/23
    PARKER, K
    COLBY, J
    BIODEGRADATION, 1995, 6 (03) : 191 - 201
  • [2] DETERMINATION OF D-2 HALOPROPIONATE DEHALOGENASE ACTIVITY FROM PSEUDOMONAS-PUTIDA STRAIN AJ1/23 BY ION CHROMATOGRAPHY
    SMITH, JM
    HARRISON, K
    COLBY, J
    TAYLOR, SC
    FEMS MICROBIOLOGY LETTERS, 1989, 57 (01) : 71 - 74
  • [3] PURIFICATION AND PROPERTIES OF 2-HALO ACID DEHALOGENASE FROM PSEUDOMONAS-PUTIDA
    MOTOSUGI, K
    ESAKI, N
    SODA, K
    AGRICULTURAL AND BIOLOGICAL CHEMISTRY, 1982, 46 (03): : 837 - 838
  • [4] PURIFICATION AND CHARACTERIZATION OF FUMARASE FROM PSEUDOMONAS-PUTIDA
    LAMARTINIERE, CA
    BRAYMER, HD
    LARSON, AD
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1970, 141 (01) : 293 - +
  • [5] PURIFICATION AND CHARACTERIZATION OF METHIONINASE FROM PSEUDOMONAS-PUTIDA
    ITO, S
    NAKAMURA, T
    EGUCHI, Y
    JOURNAL OF BIOCHEMISTRY, 1976, 79 (06): : 1263 - 1272
  • [6] NUCLEOTIDE-SEQUENCE OF THE STRUCTURAL GENE ENCODING A 2-HALOALKANOIC ACID DEHALOGENASE OF PSEUDOMONAS-PUTIDA STRAIN-AJ1 AND PURIFICATION OF THE ENCODED PROTEIN
    JONES, DHA
    BARTH, PT
    BYROM, D
    THOMAS, CM
    JOURNAL OF GENERAL MICROBIOLOGY, 1992, 138 : 675 - 683
  • [7] Insights into the molecular mechanism of dehalogenation catalyzed by D-2-haloacid dehalogenase from crystal structures
    Yayue Wang
    Yanbin Feng
    Xupeng Cao
    Yinghui Liu
    Song Xue
    Scientific Reports, 8
  • [8] PURIFICATION AND CHARACTERIZATION OF CUTINASE FROM A FLUORESCENT PSEUDOMONAS-PUTIDA BACTERIAL STRAIN ISOLATED FROM PHYLLOSPHERE
    SEBASTIAN, J
    KOLATTUKUDY, PE
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1988, 263 (01) : 77 - 85
  • [9] Purification, crystallization and preliminary crystallographic analysis of DehI, a group I α-haloacid dehalogenase from Pseudomonas putida strain PP3
    Schmidberger, Jason W.
    Wilce, Jackie A.
    Weightman, Andrew J.
    Wilce, Matthew C. J.
    ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2008, 64 : 596 - 598
  • [10] CLONING AND SEQUENCE-ANALYSIS OF A PLASMID-ENCODED 2-HALOACID DEHALOGENASE GENE FROM PSEUDOMONAS-PUTIDA NO-109
    KAWASAKI, H
    TOYAMA, T
    MAEDA, T
    NISHINO, H
    TONOMURA, K
    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1994, 58 (01) : 160 - 163
12345