DIRECT LIQUID-CHROMATOGRAPHIC SEPARATION OF 2,3-DIHYDRO-2-ETHYLBENZOFURAN-2-CARBOXYLIC ACID ENANTIOMERS USING A CELLULOSE CARBAMATE-BASED CHIRAL STATIONARY-PHASE COLUMN

Direct liquid chromatographic separation of 2,3-dihydro-2-ethylbenzofuran-2-carboxylic acid enantiomers on a commercial cellulose carbamate-based chiral stationary phase column is described. The addition of a small amount of organic acid in the mobile phase is required for the elution and separation of both enantiomers. Better peak shapes are obtained when trifluoroacetic acid is used. The method allows the detection of the undesired (+)-enantiomer down to a level of 0.5 % and can be used to measure the enantiomeric purity of (-)-enantiomer after resolution.