ESTIMATION OF PROCOLLAGEN-III AMINOPROPEPTIDE BY MEANS OF SOLID-PHASE ASSAY INVOLVING CONJUGATES OF ANTIBODIES AND PEROXIDASE OR IODINE-LABELED PROTEIN-A

A solid-phase assay was developed for estimation of procollagen III aminopropeptide which involved both immunoenzymatic and radioimmune procedures. The assay did not require the iodine-labelled aminopropeptide, while content of the antigen was estimated using the conjugate of secondary antibodies and peroxidase or iodine-labelled protein A. Substitution of the protein A for the antibodies conjugate and peroxidase enabled to abolish the radioactive materials use.