CHARACTERIZATION OF TRIACYLGLYCEROLS IN THE SEEDS OF AQUILEGIA-VULGARIS BY CHROMATOGRAPHIC AND MASS-SPECTROMETRIC METHODS

A combination of analytical techniques is generally necessary to properly characterize complex lipid materials. Chromatographic separation in conjunction with spectroscopic characterization was utilized for the analysis of the triacylglycerols in the seeds of Aquilegia vulgaris. Reversed-phase high-performance liquid chromatography (HPLC), micropacked argentation supercritical fluid chromatography (SFC), and combinations of the two techniques were used. The fatty acid profile was determined by gas chromatography/mass spectrometry of the picolinyl esters and by gas chromatography/flame-ionization detection of the methyl esters. The major components were also identified by direct inlet mass spectrometry. The excellent selectivity of packed fused silica argentation SFC for the separation of triacylglycerols was demonstrated.