MICROPROPAGATION OF JUVENILE AND ADULT SORBUS-DOMESTICA L

被引:33
|
作者
ARRILLAGA, I [1 ]
MARZO, T [1 ]
SEGURA, J [1 ]
机构
[1] UNIV VALENCIA, FAC FARM, DEPT BIOL VEGETAL, AVDA BLASCO IBANEZ 13, E-46010 VALENCIA, SPAIN
关键词
ROOTING; SHOOT PROLIFERATION; TISSUE CULTURE;
D O I
10.1007/BF00157600
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Successful propagation of seedlings and mature trees of Sorbus domestica L. has been achieved by in vitro methods. Multiple shoot formation was obtained by placing shoot apices or nodal segments on a modified Schenck and Hildebrandt medium containing benzyladenine. Regenerated shoots were excised and induced to root on media with auxin. In the best treatments 75-85% of shoots from juvenile material rooted. Rooting capacity of shoots from mature explants was lower (30%) and was not improved by dipping the base of shoots in concentration solutions of indolebutyric or naphthaleneacetic acids. Plantlets were ultimately established in soil.
引用
收藏
页码:341 / 348
页数:8
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