PTCL2-/4 - A METHIONINE-SPECIFIC LABEL FOR PROTEIN CRYSTALLOGRAPHY

被引：64
作者：
DICKERSON, RE
EISENBERG, D
VARNUM, J
KOPKA, ML
机构：
[1] Norman W. Church Laboratory, Chemical Biology California Institute of Technology, Pasadena, CA
来源：
JOURNAL OF MOLECULAR BIOLOGY | 1969年 / 45卷 / 01期
基金：
美国国家科学基金会;
关键词：
D O I：
10.1016/0022-2836(69)90210-1
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
PtCl42- has been found in six crystalline proteins to bind selectively to methionine side groups. X-ray evidence in horse heart ferricytochrome c suggests that the square-planar PtII is oxidized to octahedral PtIV with the methionine sulfur acting as a fifth ligand by donating either one or the other of its lone electron pairs in two mutually exclusive Pt binding positions. Evolutionary criteria are proposed for choosing proteins with a high probability of specific heavy-atom labeling at Met, Cys and His. © 1969.
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页码：77 / +
页数：1
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