Effects of Chlorophyll in Papaya Leaves on Superoxide Dismutation and Blood Glucose Level of Diabetic Rats

Hyperglycemia in diabetes mellitus results in oxidative stress and increases complication development. Experimental studies have shown that chlorophyll has antioxidant activity and papaya leaves contained chlorophyll more than the other green vegetables. This study aimed to evaluate the antioxidant and hypoglycemic role in chlorophyll rich in papaya leaves on diabetic rats. Thirty six rats were randomly divided into 4 groups: without treatment (technique control/TC), diabetic (negative control/NC), diabetic with treatment A (100.3 mg/200 g BW of extract) and diabetic with treatment B (200.6 mg/200 g BW of extract). Diabetic induction was conducted by injecting streptozotocin 40 mg/kg BW intraperitoneally. Extract was given by nasogastric tube. Blood glucose level was measured using enzymatic colorimetric GOD-PAP test at before, after 20 and 40 days of treatment. Hepatic superoxide dismutation (SOD) level was measured after 40 days of treatment. Blood glucose levels in 3 diabetic groups were significantly raised after seven days of induction. In Anova and post hoc LSD analysis, both treatments had lower hepatic SOD level than TC (p = 0.01), and blood glucose level also decreased after given the treatment (p = 0.01). Treatment B had a better antioxidant and hypoglycemic role than treatment A.