STUDY OF THE ANTIGENIC STRUCTURE OF VENEZUELAN EQUINE ENCEPHALOMYELITIS VIRUS GLYCOPROTEIN-E2 USING RAT MONOCLONAL-ANTIBODIES

被引：0
|
作者：
RAZUMOV, IA [1 ]
AGAPOV, EV [1 ]
PEREBOEV, AV [1 ]
PROTOPOPOVA, EV [1 ]
LEBEDEVA, SD [1 ]
LOKTEV, VB [1 ]
机构：
[1] VEKTOR SCI IND ASSOC,KOLTSOV,USSR
来源：
VOPROSY VIRUSOLOGII | 1991年 / 36卷 / 01期
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D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A collection of 21 rat hybridomas secreting highly affinic monoclonal antibodies to Venezuelan equine encephalomyelitis (VEE) virus was generated. Using a panel of 15 monoclonal antibodies to glycoprotein E2, the antigenic structure of this protein of VEE strains TC-83 and 230 was studied. A competitive radioimmunoassay suggested a new map of the antigenic structure of glycoprotein E2 in which 5 sites including 11 epitopes of monoclonal antibody binding were distinguished. Antibody to E2-2 site neutralized virus infectivity and blocked hemagglutination test and antibody to E2-3 site could only block hemagglutination. Antibodies to other E2 protein sites lacked any biological activity.
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页码：34 / 37
页数：4
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